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GAS5 rs145204276 Ins/Del Polymorphism Is Associated with CRC Susceptibility in a Romanian Population
Cecil Sorin Mirea1,2, Michael Schenker3, Bianca Petre-Mandache4
1Department of Surgery, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania.
International Journal of Molecular Sciences
|April 17, 2025
Summary
The GAS5 rs145204276 polymorphism is linked to a higher risk of colorectal cancer (CRC), especially in distal tumors and advanced stages. The CASC8 rs10505477 polymorphism showed no association with CRC risk in this Romanian population.
Area of Science:
- Genetics and Epigenetics
- Cancer Research
- Molecular Biology
Background:
- Colorectal cancer (CRC) is a significant health concern, influenced by genetic and epigenetic factors.
- Long non-coding RNAs (lncRNAs), including GAS5 and CASC8, are increasingly recognized for their roles in cancer development.
- Understanding the genetic basis of CRC susceptibility is crucial for early detection and prevention strategies.
Purpose of the Study:
- To investigate the association between GAS5 rs145204276 ins/del and CASC8 rs10505477 A>G polymorphisms and colorectal cancer (CRC) risk.
- To evaluate the potential role of these lncRNA polymorphisms in CRC susceptibility within a Romanian population.
- To identify specific CRC subtypes or stages associated with these genetic variations.
Main Methods:
- A case-control study was conducted with 156 CRC patients and 195 healthy controls.
- Genotyping of GAS5 rs145204276 and CASC8 rs10505477 polymorphisms was performed using real-time PCR.
- Logistic regression analysis was employed to calculate odds ratios (OR) and 95% confidence intervals (CI) for CRC risk.
Main Results:
- The GAS5 rs145204276 deletion allele was significantly associated with an increased risk of CRC (OR: 2.13, 95% CI: 1.24-3.63, p=0.005) under a dominant model.
- This association was particularly evident in distal colon cancer, advanced tumor stages (III+IV), and poorly differentiated tumors (G3).
- No significant association was found between the CASC8 rs10505477 A>G polymorphism and CRC risk in the studied cohort.
Conclusions:
- The GAS5 rs145204276 polymorphism may contribute to colorectal cancer susceptibility, especially in specific tumor locations and advanced stages.
- The CASC8 rs10505477 polymorphism does not appear to be a significant risk factor for CRC in the Romanian population studied.
- Further research is warranted to elucidate the functional mechanisms of GAS5 in CRC pathogenesis.
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