Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K
Notch Signaling Pathway03:14

Notch Signaling Pathway

4.1K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
4.1K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.0K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.0K
Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

5.0K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
5.0K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

2.4K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
2.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hydrophobic Refinement of Polarity-Switchable Lipo-Xenopeptides Modulates Endosomal Escape and Enhances mRNA Delivery In Vitro and In Vivo.

Journal of the American Chemical Society·2026
Same author

Homoharringtonine (omacetaxine mepesuccinate) limits the angiogenic capacity of endothelial cells and reorganises filamentous actin.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2025
Same author

Chemical Proteomics Reveals Human Off-Targets of Fluoroquinolone Induced Mitochondrial Toxicity.

Angewandte Chemie (International ed. in English)·2025
Same author

Design and Characterization of a Micro RNA-200c Detecting Broccoli Fluorescent Light-up Aptamer.

Chembiochem : a European journal of chemical biology·2025
Same author

Cellular EMT-status governs contact guidance in an electrospun TACS-mimicking <i>in vitro</i> model.

Materials today. Bio·2025
Same author

CDK5 interacts with MST2 and modulates the Hippo signalling pathway.

FEBS open bio·2024

Related Experiment Video

Updated: May 11, 2025

In Vitro Model Integrating Substrate Stiffness and Flow to Study Endothelial Cell Responses
08:53

In Vitro Model Integrating Substrate Stiffness and Flow to Study Endothelial Cell Responses

Published on: July 19, 2024

383

Endothelial Sprout Formation Is Regulated by Substrate Stiffness and Notch Signaling.

Maibritt Kretschmer1, Angelika M Vollmar1, Stefan Zahler1

  • 1Department of Pharmacy, Ludwig-Maximilians-University, Butenandtstr. 5, 81377 Munich, Germany.

International Journal of Molecular Sciences
|April 17, 2025
PubMed
Summary
This summary is machine-generated.

Notch signaling

Keywords:
Dll4Notchangiogenesisstiffness

More Related Videos

Microfluidic Model to Mimic Initial Event of Neovascularization
10:01

Microfluidic Model to Mimic Initial Event of Neovascularization

Published on: April 10, 2021

4.5K
Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels
07:49

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Published on: January 14, 2021

3.4K

Related Experiment Videos

Last Updated: May 11, 2025

In Vitro Model Integrating Substrate Stiffness and Flow to Study Endothelial Cell Responses
08:53

In Vitro Model Integrating Substrate Stiffness and Flow to Study Endothelial Cell Responses

Published on: July 19, 2024

383
Microfluidic Model to Mimic Initial Event of Neovascularization
10:01

Microfluidic Model to Mimic Initial Event of Neovascularization

Published on: April 10, 2021

4.5K
Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels
07:49

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Published on: January 14, 2021

3.4K

Area of Science:

  • Cell biology
  • Biomedical engineering
  • Vascular biology

Background:

  • Angiogenesis, or vessel formation, is regulated by the extracellular matrix's stiffness.
  • Notch signaling is a crucial pathway in angiogenesis and has been shown to respond to substrate stiffness in endothelial cells.

Purpose of the Study:

  • To investigate the role of Notch signaling in angiogenesis-related in vitro assays.
  • To examine the relationship between Notch signaling and the mechanical properties of the endothelial microenvironment.

Main Methods:

  • Utilized VEGF and Notch inhibitors as perturbations in angiogenesis assays.
  • Assessed endothelial cell behavior in tube formation and spheroid sprouting assays on substrates of varying stiffness.

Main Results:

  • Tube formation on Matrigel was influenced by substrate stiffness, but Notch signaling played a minor role.
  • Spheroid sprouting was affected by both stiffness and Notch signaling; reduced stiffness correlated with increased sprouting and Notch signaling.

Conclusions:

  • Notch signaling's role in angiogenesis is context-dependent, influencing spheroid sprouting but not tube formation on Matrigel.
  • Decreasing matrix stiffness enhances both spheroid sprouting and Notch signaling, indicating a functional link between mechanosensitivity and this pathway.