Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Radiological Investigation I: X-ray and CT01:30

Radiological Investigation I: X-ray and CT

171
Radiological investigations, including X-rays and computed tomography (CT) scans, are critical for diagnosing and evaluating various medical conditions. These imaging techniques provide valuable insights into the body's internal structures, aiding in the detection of abnormalities, assessment of disease progression, and development of treatment strategies. This article delves into two primary radiological investigations, chest X-rays and CT scans, outlining their purpose, procedures, and...
171

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Real-world effectiveness and safety of intranasal esketamine for treatment-resistant depression: data from the enTRD registry.

European psychiatry : the journal of the Association of European Psychiatrists·2026
Same author

Correction: Association of sarcopenia with survival and treatment response in brain metastasis of non-small cell lung cancer.

Scientific reports·2026
Same author

Impact of virtual monoenergetic images on the assessability of lower extremity arteries in (Poly-) trauma photon-counting detector CT.

Emergency radiology·2026
Same author

Impact of Deep-Learning Reconstruction on MRI Workflows: A Retrospective Analysis at a Large Academic Tertiary Center.

RoFo : Fortschritte auf dem Gebiete der Rontgenstrahlen und der Nuklearmedizin·2026
Same author

Clinicopsychological stratification of recovery outcomes in cerebral aneurysm patients following cluster analysis.

PNAS nexus·2026
Same author

Milnacipran continuation during pregnancy in a patient with severe post-traumatic stress disorder and psychotic depression: a case report with 3-year pediatric follow-up.

Frontiers in psychiatry·2026
Same journal

RETRACTED: Kim et al. The Angiogenesis Inhibitor ALS-L1023 from Lemon-Balm Leaves Attenuates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease Through Regulating the Visceral Adipose-Tissue Function. <i>Int. J. Mol. Sci.</i> 2017, <i>18</i>, 846.

International journal of molecular sciences·2026
Same journal

Correction: Mahmud et al. Thymoquinone Attenuates NF-κβ Signalling Activation in Retinal Pigment Epithelium Cells Under AMD-Mimicking Conditions. <i>Int. J. Mol. Sci.</i> 2025, <i>26</i>, 11473.

International journal of molecular sciences·2026
Same journal

Correction: Borovikov et al. The Twisting and Untwisting of Actin and Tropomyosin Filaments Are Involved in the Molecular Mechanisms of Muscle Contraction, and Their Disruption Can Result in Muscle Disorders. <i>Int. J. Mol. Sci</i>. 2025, <i>26</i>, 6705.

International journal of molecular sciences·2026
Same journal

Correction: Molagoda et al. Flavonoid Glycosides from <i>Ziziphus jujuba</i> var. <i>inermis</i> (Bunge) Rehder Seeds Inhibit α-Melanocyte-Stimulating Hormone-Mediated Melanogenesis. <i>Int. J. Mol. Sci.</i> 2021, <i>22</i>, 7701.

International journal of molecular sciences·2026
Same journal

Correction: Guo et al. Integrated Transcriptomic and Metabolomic Analysis Reveals the Molecular Regulatory Mechanism of Flavonoid Biosynthesis in Maize Roots Under Lead Stress. <i>Int. J. Mol. Sci.</i> 2024, <i>25</i>, 6050.

International journal of molecular sciences·2026
Same journal

Correction: Chang et al. Improvement of Carbon Tetrachloride-Induced Acute Hepatic Failure by Transplantation of Induced Pluripotent Stem Cells Without Reprogramming Factor c-Myc. <i>Int. J. Mol. Sci.</i> 2012, <i>13</i>, 3598-3617.

International journal of molecular sciences·2026
See all related articles

Related Experiment Video

Updated: May 11, 2025

Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics
09:05

Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics

Published on: June 29, 2020

5.1K

Routine CT Diagnostics Cause Dose-Dependent Gene Expression Changes in Peripheral Blood Cells.

Hanns Leonhard Kaatsch1,2, Laura Kubitscheck2, Simon Wagner2

  • 1Department of Radiology and Neuroradiology, Bundeswehr Central Hospital, 56072 Koblenz, Germany.

International Journal of Molecular Sciences
|April 17, 2025
PubMed
Summary
This summary is machine-generated.

Computed tomography (CT) scans can alter gene expression in blood cells, with effects increasing with radiation dose. AEN gene shows promise as a biomarker for CT exposure, supporting the ALARA principle for radiation safety.

Keywords:
computed tomographygene expressionlow-dose radiationrisk assessmentwhole transcriptome sequencing

More Related Videos

Expedited Radiation Biodosimetry by Automated Dicentric Chromosome Identification ADCI and Dose Estimation
10:33

Expedited Radiation Biodosimetry by Automated Dicentric Chromosome Identification ADCI and Dose Estimation

Published on: September 4, 2017

15.6K
Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
12:36

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Published on: June 26, 2018

9.3K

Related Experiment Videos

Last Updated: May 11, 2025

Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics
09:05

Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics

Published on: June 29, 2020

5.1K
Expedited Radiation Biodosimetry by Automated Dicentric Chromosome Identification ADCI and Dose Estimation
10:33

Expedited Radiation Biodosimetry by Automated Dicentric Chromosome Identification ADCI and Dose Estimation

Published on: September 4, 2017

15.6K
Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
12:36

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Published on: June 26, 2018

9.3K

Area of Science:

  • Medical Imaging
  • Molecular Biology
  • Radiation Oncology

Background:

  • Computed tomography (CT) use is rising, increasing cumulative radiation exposure and raising concerns about long-term health effects.
  • Epidemiological studies link CT examinations to higher tumor incidence, but biological mechanisms are unclear.
  • Understanding cellular responses to diagnostic radiation is crucial for risk assessment.

Purpose of the Study:

  • Investigate CT-induced gene expression changes in peripheral blood cells.
  • Identify potential biomarkers for CT exposure.
  • Assess the dose-dependent effects of CT radiation on gene expression.

Main Methods:

  • Whole transcriptome sequencing of peripheral blood cells from 40 male patients before and after multi-phase abdominal CT.
  • Differential gene expression analysis using DESeq2.
  • Analysis of gene expression changes across low, medium, and high radiation dose categories.

Main Results:

  • CT exposure induced a dose-dependent upregulation of six genes (EDA2R, AEN, FDXR, DDB2, PHLDA3, MIR34AHG), all linked to TP53 regulation and DNA damage response.
  • Gene Set Enrichment Analysis indicated increased cellular damage and metabolic changes, potentially an adaptive response to low-dose irradiation.
  • AEN was identified as the most consistent biomarker for CT exposure, regardless of dose.

Conclusions:

  • Routine abdominal CT scans cause dose-dependent gene deregulation and DNA damage in peripheral blood cells.
  • The findings support the "As Low As Reasonably Achievable" (ALARA) principle for CT radiation risk management.
  • Complex molecular responses, including metabolic changes beyond DNA damage, highlight the need for further research into CT exposure effects.