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Updated: May 11, 2025
13:18
Single Oocyte Bisulfite Mutagenesis
Published on: June 27, 2012
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DNA Methylation Patterns and Transcriptomic Data Were Integrated to Investigate Candidate Genes Influencing
Lin Ma1, Xianzhi Zhao1, Haiwei Wang1
1Chongqing Engineering Research Center of Goose Genetic Improvement, Institute of Poultry Science, Chongqing Academy of Animal Science, Chongqing 402460, China.
International Journal of Molecular Sciences
|April 17, 2025
Summary
DNA methylation influences goose reproductive traits. High-laying geese show more hypermethylated regions, with EML6 identified as a key gene for follicle development and breeding potential.
Area of Science:
- Animal Genetics
- Epigenetics
- Reproductive Biology
Background:
- Ovarian function is crucial for goose reproduction.
- DNA methylation plays a significant role in regulating gene expression and biological processes.
- Understanding epigenetic modifications in goose ovaries can reveal insights into reproductive performance.
Purpose of the Study:
- To investigate the role of DNA methylation in goose reproductive traits.
- To identify differentially methylated genes and pathways associated with laying rate.
- To explore the relationship between DNA methylation and gene expression in goose ovarian tissue.
Main Methods:
- Whole-genome bisulfite sequencing (WGBS) was performed on ovarian tissues from high-laying-rate geese (HLRG) and low-laying-rate geese (LLRG).
- Differentially methylated regions (DMRs) and differentially methylated genes (DMGs) were identified.
- Gene Ontology (GO) and pathway enrichment analyses were conducted.
- Integration with RNA-sequencing (RNA-seq) data was used to identify overlapping genes between DMGs and differentially expressed genes (DEGs).
Main Results:
- HLRG exhibited a higher level of hypermethylated DMRs compared to LLRG.
- A total of 2831 DMRs and 733 DMGs were identified, with 363 genes showing upregulated methylation.
- Enriched pathways included microtubule function, GnRH secretion, thyroid hormone signaling, ECM-receptor interaction, and PI3K-Akt signaling.
- Eight overlapping genes between DMGs and DEGs were found, with five genes (CUL9, MEGF6, EML6, SYNE2, AK1BA) showing hypomethylation correlated with high expression.
- The gene EML6 was highlighted as a potential regulator of follicle growth and development.
Conclusions:
- DNA methylation is a key regulatory mechanism influencing reproductive traits in geese.
- Specific genes, notably EML6, are identified as potential targets for improving goose breeding.
- This study provides valuable insights and candidate markers for enhancing goose reproductive performance through genetic and epigenetic approaches.
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