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Retinal to Retinal Energy Transfer in a Bistable Microbial Rhodopsin Dimer.

Ivo H M van Stokkum1, Jakub Dostal2, Thanh Nhut Do1

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Summary
This summary is machine-generated.

Neorhodopsin (NeoR) is a fungal protein that switches between UV and near-IR states. Researchers found an energy transfer process between its chromophores, crucial for its function.

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Area of Science:

  • Biophysics
  • Photochemistry
  • Structural Biology

Background:

  • Neorhodopsin (NeoR) is a novel fungal bistable rhodopsin.
  • It exhibits reversible photoswitching between UV-absorbing (NeoR367) and near-IR-absorbing (NeoR690) states.
  • NeoR forms homodimers with closely spaced retinal chromophores.

Purpose of the Study:

  • To investigate the excited-state dynamics of Neorhodopsin.
  • To elucidate the mechanism and kinetics of excitation energy transfer (EET) between chromophores in NeoR dimers.
  • To understand the role of intramolecular charge-transfer (ICT) states in NeoR function.

Main Methods:

  • Ultrafast spectroscopy to probe excited-state relaxation.
  • Cryo-electron microscopy (Cryo-EM) for structural analysis.
  • Computational modeling to assess EET rates under varying photoequilibrium conditions.

Main Results:

  • UV excitation of NeoR367 leads to rapid internal conversion to the S1 state.
  • An intradimer EET process from NeoR367 (S1) to NeoR690 was observed.
  • The EET rate was determined to be (200 ps)⁻¹.
  • A weak stimulated emission band in NeoR367 (S1) suggests mixing with an ICT state.

Conclusions:

  • Excitation energy transfer (EET) is a key process in Neorhodopsin's excited-state dynamics.
  • EET occurs between retinal chromophores within NeoR homodimers.
  • This EET mechanism may be relevant for the function of other bistable, multiwavelength rhodopsins.