Related Experiment Video
Updated: Jul 16, 2026

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples
Published on: December 22, 2014
High-throughput community and metagenomic elucidate systematic performance variation and functional transition
Rushuo Yang1, Zhe Liu2, Yongjun Liu2
1School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Yan Ta Road. No.13, Xi'an 710055, China.
Abstract:
In this study, high-throughput sequencing and metagenomics were used to investigate the microbial succession and functional gene dynamics during aerobic sludge granulation from activated sludge (AS) to aerobic granular sludge (AGS) to algal-bacterial granular sludge (ABGS). It was found that the settleability and pollutant removal efficiency of the sludge system increased with the sludge morphology evolution. Extracellular polymeric substances (EPS) analysis showed a rise in protein from 2.1 to 17.4 mg/gSS during stage of AGS and polysaccharides from 3.3 to 5.9 mg/gSS during stage of ABGS. Microbial community analysis revealed that the sludge evolution reduced species richness but enriched functional bacteria for nitrogen/phosphorus removal, while increasing the complexity of community structure and close interactions between species. Key genes involved in the tricarboxylic acid cycle, nitrogen/phosphorus and EPS metabolism were also upregulated. This study revealed the continuity mechanism and stage dependence of the functional transition during sludge morphology evolution.
More Related Videos
09:57Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities
Published on: July 12, 2018
09:38Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
Published on: January 7, 2019
Related Concept Videos
Modern Molecular Taxonomy
Evolution of New Traits in Microbes
Methods to Assess Microbial Communities
Microbial Mats
Deep Sea Microbial Ecology
Microbial Wastewater Treatment