Recent advances in bioassays for assessing the toxicity of environmental contaminants in effect-directed analysis

Chemical cocktails in the environment can cause adverse impacts on ecosystems and human health even at low concentrations. Effect-directed analysis (EDA) has proven to be very valuable in identifying key toxic substances in environmental mixtures. For this, it is important to carefully select accurate bioassays from a wide range of tests for EDA when applying it to actual environmental samples. This article reviews studies published from 2014 to 2023 that have applied EDA and summarizes the bioassays and their corresponding biological effects. A total of 127 studies were selected from 591 publications evaluating the toxic effects of environmental samples, including wastewater, surface water, and sediments. Here, bioassays used in EDA are summarized, including the assays that measure specific receptor-mediated modes of action (MOA), induction of xenobiotic metabolism pathways, and induction of adaptive stress response pathways using either in vitro or in vivo bioassays. Also, the identified substances using EDA are discussed based on their MOA. The importance of EDA in establishing a comprehensive approach for the detection of environmental contaminants using bioanalytical methods is emphasized. The current limitations and benefits of using EDA in practical applications are outlined and strategies for moving forward are proposed.