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Updated: May 11, 2025

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
Published on: September 20, 2016
Advancing yeast metabolism for a sustainable single carbon bioeconomy
Miriam Kuzman1,2, Özge Ata1,2, Diethard Mattanovich1,2
1Institute of Microbiology and Microbial Biotechnology, Department of Biotechnology and Food Science, BOKU University, 1190, Vienna, Austria.
Abstract:
Single carbon (C1) molecules are considered as valuable substrates for biotechnology, as they serve as intermediates of carbon dioxide recycling, and enable bio-based production of a plethora of substances of our daily use without relying on agricultural plant production. Yeasts are valuable chassis organisms for biotech production, and they are able to use C1 substrates either natively or as synthetic engineered strains. This minireview highlights native yeast pathways for methanol and formate assimilation, their engineering, and the realization of heterologous C1 pathways including CO2, in different yeast species. Key features determining the choice among C1 substrates are discussed, including their chemical nature and specifics of their assimilation, their availability, purity, and concentration as raw materials, as well as features of the products to be made from them.
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