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Updated: Aug 25, 2026

Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays
Published on: April 4, 2011
Natural heterogeneity of shape, infectivity and protein composition in an influenza A (H3N2) virus preparation
Abstract:
Influenza A (X31) virus was purified over a zonal sucrose gradient. The resulting gradient fractions were examined by electron microscopy, HA and infectivity titrations and gel electrophoresis. The fractions containing a homogeneous suspension of spherical particles had the highest infectivity per amount of viral protein and a much higher HA:M ratio than the unfractionated preparation. These results explain differences in the proportions of HA and M protein we have reported elsewhere on monodisperse virus suspensions compared with earlier measurements on crude suspensions.
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