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Adsorption, Structure, and Dynamics of DNA in Montmorillonite and Montmorillonite-Humic Acid: A Molecular-Level
Fayang Guo1, Mengqi Shi1, Yuxiang Mao1
1Institute of Resources & Environment, Henan Polytechnic University, Jiaozuo 454000, China.
Langmuir : the ACS Journal of Surfaces and Colloids
|April 18, 2025
Summary
Extracellular DNA (eDNA) adsorption on soil minerals is key for public health. Humic acids reduce DNA binding to montmorillonite, impacting eDNA stability and migration in soils.
Area of Science:
- Environmental Science
- Soil Science
- Biogeochemistry
Background:
- Extracellular DNA (eDNA) in soil is crucial for public health.
- Soil clay minerals and organic matter control eDNA mobility and stability.
- Molecular mechanisms of DNA adsorption on montmorillonite-humic acid (MMT-HA) complexes are unclear.
Purpose of the Study:
- To investigate the molecular interactions governing DNA adsorption and diffusion on MMT and MMT-HA complexes.
- To elucidate the role of humic acids in modifying DNA-mineral interactions.
Main Methods:
- Molecular dynamics simulations were employed.
- Analysis included interaction energy, diffusion coefficients, and interfacial structure.
Main Results:
- DNA adsorption is more favorable on MMT than on MMT-HA.
- Humic acids decrease DNA adsorption capacity on MMT.
- DNA mobility is lower on MMT compared to MMT-HA.
- Ca2+ cations and water bridge DNA to MMT, enhancing adsorption on MMT alone.
Conclusions:
- Humic acids inhibit DNA adsorption on MMT by occupying binding sites and limiting hydrogen bonds.
- Understanding these interactions is vital for predicting eDNA fate in soil environments.
- Results offer insights into eDNA stability and migration in soils.
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