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Related Experiment Video

Updated: May 11, 2025

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Rab11b is necessary for mitochondrial integrity and function in gut epithelial cells.

Ivor Joseph1, Jiangmeng Han1, Jared Bianchi-Smak1

  • 1Department of Biological Sciences, Rutgers University, Newark, NJ, United States.

Frontiers in Cell and Developmental Biology
|April 18, 2025
PubMed
Summary
This summary is machine-generated.

The small GTPase RAB11B plays a novel role in maintaining mitochondrial structure and function. This study reveals RAB11B

Keywords:
Paneth cellRab11intestinemitochondriaproteomics

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Mitochondrial Biology

Background:

  • RAB11 family small GTPases regulate intracellular trafficking.
  • RAB11A and RAB11B redundantly control spindle dynamics in gut epithelial cells.
  • The specific functions of RAB11B beyond RAB11A are not well understood.

Purpose of the Study:

  • To investigate the distinct cellular functions of RAB11B.
  • To explore RAB11B's role in regulating mitochondrial integrity and function.

Main Methods:

  • Proteomic analysis of RAB11A and RAB11B interactomes.
  • Transcriptomic analysis of Rab11b knockout mouse intestines.
  • Flow cytometry for mitochondrial membrane potential and ROS.
  • Electron microscopy of Paneth cells.

Main Results:

  • Proteomics suggested RAB11B interacts with mitochondrial proteins.
  • Rab11b knockout intestines showed altered mitochondrial protein targeting and function.
  • Impaired mitochondrial membrane potential and increased ROS were observed in vivo.
  • Severe mitochondrial membrane defects were found in Paneth cells of knockout mice.

Conclusions:

  • RAB11B is linked to mitochondrial structural maintenance.
  • RAB11B is implicated in the functional integrity of mitochondria.
  • This study establishes a novel role for RAB11B in mitochondrial homeostasis.