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Updated: May 11, 2025
A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
Published on: March 25, 2014
Analysis of epitopes and structural responses in egg allergen Gal d 1 using bioinformatic tools and molecular
Tao Wang1, Lili Zhang1, Vijaya Raghavan2
1Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, and Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing, 210009, China.
Abstract:
Egg allergy is a growing concern worldwide. Investigating the allergenic and molecular characteristics of egg allergen Ovomucoid (Gal d 1) can enhance our understanding of egg allergies. In this study, the B-cell linear epitopes of Gal d 1 were predicted by using different bioinformatic tools, based on the primary sequence properties of Gal d 1, and obtained 10 potential B-cell linear epitopes. Meanwhile, we conducted molecular dynamics (MD) simulations to apply thermal and an oscillating electric field treatments to Gal d 1 in order to comprehend the structural alterations of Gal d 1 intuitively. The results indicated that Gal d 1 was a thermally stable protein, while the secondary structure and surface characteristics of Gal d 1 were obviously influenced by the combination of thermal stress and electric field, which finally resulted in conformational changes. This study provided a new way to understand the development of hypoallergenic egg products.
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