Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Responses to Heat and Cold Stress02:45

Responses to Heat and Cold Stress

13.2K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.2K
Responses to Drought and Flooding02:41

Responses to Drought and Flooding

10.5K
Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.
10.5K
Adaptations that Reduce Water Loss01:57

Adaptations that Reduce Water Loss

25.0K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
25.0K
Multipotency and Niche of Bulge Stem Cell01:06

Multipotency and Niche of Bulge Stem Cell

3.2K
A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...
3.2K
Regulation of Transpiration by Stomata02:04

Regulation of Transpiration by Stomata

27.6K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
27.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

More than a blueprint: Developmental regulators secure the cellular environment for regeneration.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Preassembly and independent trafficking of the exocyst complex in <i>Arabidopsis</i>.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Desert-adapted plant growth-promoting pseudomonads modulate plant auxin homeostasis and mitigate salinity stress.

Microbial biotechnology·2024
Same author

Symplastic communication in the root cap directs auxin distribution to modulate root development.

Journal of integrative plant biology·2022
Same author

Abnormal accumulation of lipid droplets in neurons induces the conversion of alpha-Synuclein to proteolytic resistant forms in a Drosophila model of Parkinson's disease.

PLoS genetics·2021
Same author

msGBS: A new high-throughput approach to quantify the relative species abundance in root samples of multispecies plant communities.

Molecular ecology resources·2020
Same journal

Directed Evolution of Plant-Associated Bacteria Enhances Plant Holobiont Stress Tolerance.

Plant, cell & environment·2026
Same journal

Antagonistic Regulation of the Conifer Age Marker DAL1 by GA and JA Via a TIFY25-DPL1 Module.

Plant, cell & environment·2026
Same journal

Leaf Tissue-Specific Phosphorus Allocation Is Linked to Leaf Lifespan in Chickpea Accessions.

Plant, cell & environment·2026
Same journal

Quantitative and Hierarchical Coordination of Growth and Defence in Tomato (Solanum lycopersicum L.) Under Increasing Tissue Damage.

Plant, cell & environment·2026
Same journal

FERONIA: A Central Hub Integrating Environmental Signaling and the Growth-Defense Trade-Off in Plants.

Plant, cell & environment·2026
Same journal

Systems-Level Developmental Reprogramming Under Waterlogging Stress in Cowpea Revealed by Integrated Phenotypic, Physiological, and Transcriptomic Analysis.

Plant, cell & environment·2026
See all related articles

Related Experiment Video

Updated: May 11, 2025

Geomagnetic Field Gmf and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression
11:04

Geomagnetic Field Gmf and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression

Published on: November 30, 2015

13.2K

Root Hair Development Is Suppressed by Long-Term Mild Heat Through Down-Regulation of RHD6 and RHD6-like Genes.

Gaigai Du1, Xin Tian1, Daan M van den Brink1

  • 1Department of Plant & Animal Biology, Radboud Institute for Biological and Environmental Sciences, Radboud University, Nijmegen, The Netherlands.

Plant, Cell & Environment
|April 18, 2025
PubMed
Summary
This summary is machine-generated.

Long-term mild heat stress suppresses root hair initiation in Arabidopsis thaliana by downregulating RHD6 and its homologues. This heat-induced inhibition of root hair development is GL2-independent, offering insights for improving plant heat tolerance.

Keywords:
RHD6cell fatedevelopmentheatinitiationroot hair

More Related Videos

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function
07:34

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

3.4K
Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat Fagopyrum tataricum
08:12

Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat Fagopyrum tataricum

Published on: March 11, 2020

11.9K

Related Experiment Videos

Last Updated: May 11, 2025

Geomagnetic Field Gmf and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression
11:04

Geomagnetic Field Gmf and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression

Published on: November 30, 2015

13.2K
Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function
07:34

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

3.4K
Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat Fagopyrum tataricum
08:12

Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat Fagopyrum tataricum

Published on: March 11, 2020

11.9K

Area of Science:

  • Plant Biology
  • Molecular Biology
  • Environmental Stress Physiology

Background:

  • Root hairs are crucial for plant water and nutrient uptake.
  • High temperatures in upper soil layers pose a significant stress to plant roots.
  • The specific effects of heat stress on root hair development remain largely uncharacterized.

Purpose of the Study:

  • To investigate the impact of long-term mild heat stress on root hair initiation in Arabidopsis thaliana.
  • To elucidate the molecular mechanisms underlying heat-induced suppression of root hair development.
  • To identify potential targets for enhancing plant resilience to high temperatures.

Main Methods:

  • Arabidopsis thaliana seedlings were exposed to long-term mild heat stress.
  • Analysis of root hair initiation and epidermal cell patterning using marker genes (GL2, CPC).
  • Investigated the role of RHD6 and related transcription factors (RSL2, RSL4) through gene expression analysis and overexpression studies.
  • Ethylene treatment was used to assess its effect on gene expression and root hair phenotype.

Main Results:

  • Long-term mild heat stress significantly suppressed root hair initiation in Arabidopsis.
  • Heat-induced suppression was independent of GL2 activity and upstream regulators.
  • Expression of downstream RHD6 and RHD6-like genes (RSL2, RSL4) was reduced under heat stress.
  • Overexpression of RHD6 rescued the root hair phenotype and restored RSL2/RSL4 expression.

Conclusions:

  • GL2-independent downregulation of RHD6 and its homologues mediates heat stress-induced inhibition of root hair initiation.
  • Understanding this pathway provides a basis for developing strategies to improve plant performance under high temperature conditions.
  • This research contributes to plant stress physiology and crop improvement for warmer climates.