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Updated: May 11, 2025

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
Published on: November 1, 2018
A Fast One-Step RT-qPCR to Quantify Yellow Fever Virus
Gisela Freitas Trindade1, Alice Gomes Fernandes2,3, Sheila Maria Barbosa de Lima1
1Laboratory of Virological Technology (LATEV), Institute of Technology in Immunobiologicals (Bio-Manguinhos), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil.
Abstract:
Quantitative RT-PCR (qPCR) provides a rapid, specific, and sensitive method for the determination of yellow fever viral load and presents many advantages including quantitative measurement, low contamination rate, and easy standardization. Here it is presented a semi-automatic RNA extraction followed by one-step PCR reaction. Genome copies were quantified by plasmid standard curve designed to NS5 region. This method provides a specific, rapid, and sensitive diagnosis to yellow fever virus (YFV).

