Related Experiment Video
Updated: May 11, 2025

Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus
Published on: October 1, 2015
Immunofluorescence Assay
Andréa Nazaré Monteiro Rangel da Silva1, José Valter Joaquim Silva Júnior2
1Institute of Biological Sciences, Virology Laboratory, Federal University of Pará, Belém, Pará, Brazil.
Abstract:
The investigation of yellow fever suspected cases is important for both epidemiological surveillance and for the timely adoption of supportive therapy. The immunofluorescence assay (IFA), a technique based on the detection of specific targets by fluorescent dye-tagged antibodies, may be useful for the serological diagnosis of yellow fever and/or for monitoring of viral isolation. In addition, IFA can also be used for detection of yellow fever virus (YFV) antigens, as well as for recombinant YFV proteins after cell transfection. Here we describe a simple in-house IFA protocol for YFV, which may be useful for research purposes or laboratory diagnosis.
Related Concept Videos
Immunofluorescence Microscopy
Enzyme-Linked Immunosorbent Assay
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...
Immunocytochemistry and Immunohistochemistry
These...

