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Related Experiment Video

Updated: May 11, 2025

Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus

Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus

Published on: October 1, 2015

Immunofluorescence Assay.

Andréa Nazaré Monteiro Rangel da Silva¹, José Valter Joaquim Silva Júnior²

¹Institute of Biological Sciences, Virology Laboratory, Federal University of Pará, Belém, Pará, Brazil.

Methods in Molecular Biology (Clifton, N.J.)

|April 18, 2025

Summary

Investigating suspected yellow fever cases aids surveillance and treatment. A new immunofluorescence assay (IFA) protocol offers a simple method for yellow fever virus (YFV) diagnosis and research.

Keywords:

Cell transfection monitoring Diagnosis Fluorescent dye-tagged antibody Protein detection Serology Viral isolation Yellow fever

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Area of Science:

Virology
Immunology
Epidemiology

Background:

Yellow fever (YF) surveillance is crucial for public health.
Early diagnosis and supportive therapy are vital for YF patient management.
The immunofluorescence assay (IFA) is a valuable serological tool.

Purpose of the Study:

To describe a simple in-house immunofluorescence assay (IFA) protocol for yellow fever virus (YFV).
To highlight the utility of IFA in YFV diagnosis, surveillance, and research.

Main Methods:

Development and validation of an in-house immunofluorescence assay (IFA) protocol.
Utilizing fluorescent dye-tagged antibodies for YFV detection.
Application of IFA for serological diagnosis, viral isolation monitoring, and antigen detection.

Main Results:

The described IFA protocol is simple and effective for YFV detection.
IFA can be used for serological diagnosis and monitoring viral isolation.
IFA facilitates the detection of YFV antigens and recombinant proteins.

Conclusions:

The in-house IFA protocol provides a valuable tool for yellow fever virus (YFV) research.
This method can aid in laboratory diagnosis and epidemiological surveillance of YF.