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Updated: May 11, 2025

Detection of Viral RNA by Fluorescence in situ Hybridization FISH
Published on: May 5, 2012
Detection of Viral Particles in Paraffinized Tissues and Cell Culture by Indirect Immunofluorescence
Yuli Souza1, Barbara de Oliveira1, Marcelo Pelajo-Machado1
1Laboratory of Experimental Medicine and Health (LAMES), Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, RJ, Brazil.
Abstract:
Immunofluorescence enables detection of proteins in tissue and cell culture. The specific reaction between the complex antigen-antibody confers security to detection. Disclosure of proteins of interest by fluorochrome allows a perception of its spatial distribution and location. Here we describe the technique of indirect immunofluorescence for detection of E protein from yellow fever virus in paraffinized tissue and in cell cultures.

