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Micro-Focus Reduction Neutralization Tests (mFRNT) for Neutralizing Antibody (Nab) Quantification Against Yellow

Adriana de Souza Azevedo1, Nathalia Dos Santos Alves2

  • 1Laboratory of Immunomolecular Analysis, Institute of Technology in Immunobiologicals (Bio-Manguinhos), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, Brazil. adriana.soares@bio.fiocruz.br.

Methods in Molecular Biology (Clifton, N.J.)
|April 18, 2025
PubMed

Keywords:
FlavivirusFocusImmunoassayNabNeutralizing antibodiesYellow fever virusmFRNT

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Yellow Fever01:18

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Yellow fever is a viral hemorrhagic disease caused by the yellow fever virus (YFV), a member of the Flaviviridae family. It is transmitted primarily by Aedes and Haemagogus mosquitoes in tropical and subtropical regions of Africa and South America. After transmission through a mosquito bite, the virus initially replicates in skin-resident immune cells such as dendritic cells and macrophages. These cells then migrate to the lymph nodes, where viral replication increases, eventually leading to...