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Related Experiment Video

Updated: May 3, 2026

Imaging Odor-Evoked Activities in the Mouse Olfactory Bulb using Optical Reflectance and Autofluorescence Signals
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Image-Based cAMP Sensor Assay for Analyzing Odorant Receptor Activity.

JiWoo Choi1,2, JaeHyung Koo3,4,5

  • 1Department of New Biology, DGIST , Daegu, Republic of Korea.

Methods in Molecular Biology (Clifton, N.J.)
|April 18, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a new fluorescence-based assay for real-time visualization of odorant receptor (OR) and ligand interactions. This method aids in identifying new drug targets and understanding OR function beyond the nose.

Keywords:
GPCROdorant receptorsOlfactory receptorPink FlamindocAMP sensor

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Area of Science:

  • Pharmacology
  • Molecular Biology
  • Biochemistry

Background:

  • Odorant receptors (ORs), a large family of G-protein-coupled receptors (GPCRs), are crucial for smell.
  • ORs are increasingly recognized for roles in non-olfactory tissues, presenting potential therapeutic targets.
  • Current functional analysis of ORs is limited by ligand availability and challenges in high-throughput screening.

Purpose of the Study:

  • To develop an advanced, imaging-based method for studying OR-ligand interactions.
  • To overcome limitations of conventional cAMP assays for large-scale OR functional analysis.
  • To facilitate the deorphanization of ORs and explore their non-olfactory functions.

Main Methods:

  • Utilized the Pink Flamindo sensor, a cAMP-sensitive fluorescent reporter.
  • Developed a real-time, imaging-based assay to visualize cAMP production upon OR activation.
  • Applied the assay for the functional characterization and deorphanization of odorant receptors.

Main Results:

  • Demonstrated successful real-time visualization of OR-ligand interactions via fluorescence.
  • Showcased the Pink Flamindo sensor's utility in detecting cAMP changes associated with OR activity.
  • Validated the assay's effectiveness in aiding OR deorphanization.

Conclusions:

  • The developed Pink Flamindo-based cAMP assay offers a robust platform for studying ORs.
  • This technique enhances the ability to identify OR ligands and understand their roles in various tissues.
  • The method provides a valuable tool for drug discovery targeting GPCRs, including odorant receptors.