J-Aggregated Fluorescence Nanoparticles with Multichromatic Performance Enable Sensitive LFIA Platform

Area of Science:

• Nanotechnology
• Biosensing
• Analytical Chemistry

Background:

• Conventional lateral flow immunoassays (LFIA) suffer from limited sensitivity and single signal output.
• Development of sensitive and multiplexed detection platforms is crucial for rapid on-site toxin analysis.

Purpose of the Study:

• To develop J-aggregated fluorescence nanoparticles (JNPs) as multichromatic reporters for enhanced LFIA.
• To achieve simultaneous and sensitive detection of T-2 and AFB1 toxins on a multiplexed LFIA platform.

Main Methods:

• Synthesized JNPs exhibiting a strong J-aggregation phenomenon with red-shifted emission (∼620 nm).
• Developed a multiplexed LFIA platform (JNPs-LFIA) using green and red emitting JNPs.
• Quantitatively detected T-2 and AFB1 toxins on the developed JNPs-LFIA platform.

Main Results:

• JNPs displayed red-shifted emission due to nanoparticle confinement and J-aggregation.
• JNPs-LFIA achieved limits of detection of 0.645 ng mL⁻¹ for T-2 toxin and 0.0035 ng mL⁻¹ for AFB1 toxin.
• The developed JNPs-LFIA showed 3.5-fold and 12.2-fold higher sensitivity than AuNPs-LFIA for T-2 and AFB1 toxins, respectively.

Conclusions:

• This work presents an efficient strategy for designing multichromatic immunoprobes.
• The developed JNPs-LFIA platform offers a sensitive and simultaneous detection method for T-2 and AFB1 toxins.
• This advancement promotes the development of novel reporters for advanced biosensing applications.