Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tartary Buckwheat protein-derived peptide AFYRW alleviates H₂O₂-induced inflammation and apoptosis in HaCaT cells by activating SIRT1-dependent deacetylation.

Tissue & cell·2026
Same author

Phenotyping elucidates the association between traits and bioactive components in Notopterygium incisum.

BMC plant biology·2026
Same author

Impact of CYP3A5 genotype on tacrolimus pharmacokinetics and clinical outcomes in pediatric patients with Henoch-Schönlein purpura nephritis.

Pharmacogenetics and genomics·2026
Same author

Synergistic Light-Harvesting and Heat Transfer in Platinum-Confined Gold Nanocages for Sensitive Photothermal Lateral Flow Immunoassay.

Analytical chemistry·2026
Same author

From Phage Display to Yeast Secretion: Developing Fc-Fused Nanobodies Against Influenza Virus.

Cells·2026
Same author

Characterization of a Goose-Origin Avian Orthoreovirus with Interferon Suppression Activity.

Viruses·2026

Related Experiment Video

Updated: Jun 12, 2025

Fluorescence Imaging with One-nanometer Accuracy FIONA
11:56

Fluorescence Imaging with One-nanometer Accuracy FIONA

Published on: September 26, 2014

17.6K

J-Aggregated Fluorescence Nanoparticles with Multichromatic Performance Enable Sensitive LFIA Platform.

Gan Zhang1, Keyang Lai1, Lilin Zhang2

  • 1State Key Laboratory of Food Science and Resources, Nanchang University, 330047 Nanchang, China.

Nano Letters
|April 18, 2025
PubMed
Summary
This summary is machine-generated.

We developed J-aggregated fluorescence nanoparticles (JNPs) for sensitive, simultaneous detection of T-2 and AFB1 toxins using a multiplexed lateral flow immunoassay (LFIA). This biosensing approach significantly enhances detection limits compared to conventional methods.

Keywords:
DetectionJ-aggregationLateral flow immunoassayMultichromaticToxins

More Related Videos

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

8.9K
Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

1.2K

Related Experiment Videos

Last Updated: Jun 12, 2025

Fluorescence Imaging with One-nanometer Accuracy FIONA
11:56

Fluorescence Imaging with One-nanometer Accuracy FIONA

Published on: September 26, 2014

17.6K
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

8.9K
Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

1.2K

Area of Science:

  • Nanotechnology
  • Biosensing
  • Analytical Chemistry

Background:

  • Conventional lateral flow immunoassays (LFIA) suffer from limited sensitivity and single signal output.
  • Development of sensitive and multiplexed detection platforms is crucial for rapid on-site toxin analysis.

Purpose of the Study:

  • To develop J-aggregated fluorescence nanoparticles (JNPs) as multichromatic reporters for enhanced LFIA.
  • To achieve simultaneous and sensitive detection of T-2 and AFB1 toxins on a multiplexed LFIA platform.

Main Methods:

  • Synthesized JNPs exhibiting a strong J-aggregation phenomenon with red-shifted emission (∼620 nm).
  • Developed a multiplexed LFIA platform (JNPs-LFIA) using green and red emitting JNPs.
  • Quantitatively detected T-2 and AFB1 toxins on the developed JNPs-LFIA platform.

Main Results:

  • JNPs displayed red-shifted emission due to nanoparticle confinement and J-aggregation.
  • JNPs-LFIA achieved limits of detection of 0.645 ng mL⁻¹ for T-2 toxin and 0.0035 ng mL⁻¹ for AFB1 toxin.
  • The developed JNPs-LFIA showed 3.5-fold and 12.2-fold higher sensitivity than AuNPs-LFIA for T-2 and AFB1 toxins, respectively.

Conclusions:

  • This work presents an efficient strategy for designing multichromatic immunoprobes.
  • The developed JNPs-LFIA platform offers a sensitive and simultaneous detection method for T-2 and AFB1 toxins.
  • This advancement promotes the development of novel reporters for advanced biosensing applications.