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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
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Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

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MicroRNA transcriptome analysis for post-mortem interval estimation.

Haneul Lee1, Eun Ju Lee2, Kwangmin Park3

  • 1Department of Forensic Science, Graduate school, Catholic University of Pusan, Busan 46252, Republic of Korea; Next-Generation Industrial Field-Based Specialist Program for Molecular Diagnostics, Brain Busan 21 Plus Project, Graduate School, Catholic University of Pusan, Republic of Korea.

Forensic Science International
|April 18, 2025
PubMed
Summary
This summary is machine-generated.

This study explored microRNAs (miRNAs) as biomarkers for estimating the post-mortem interval (PMI). Specific miRNA expression levels correlated with PMI, offering potential for more accurate forensic analysis.

Keywords:
Forensic biomarkersGene ontology analysisMicroRNAPost-mortem interval

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Area of Science:

  • Forensic Science
  • Molecular Biology
  • Genomics

Background:

  • Accurate post-mortem interval (PMI) estimation is crucial in forensic investigations.
  • Current PMI estimation methods face limitations due to variability in influencing factors.
  • MicroRNAs (miRNAs) are being investigated as stable molecular biomarkers for forensic applications.

Purpose of the Study:

  • To evaluate the utility of microRNAs (miRNAs) as reliable biomarkers for estimating the post-mortem interval (PMI).
  • To identify specific miRNA expression profiles associated with different post-mortem intervals.
  • To explore the potential of miRNAs in enhancing the accuracy of forensic PMI determination.

Main Methods:

  • Analysis of miRNA expression profiles in mouse myocardial tissue across six distinct post-mortem intervals (0 to 6 days).
  • Utilized high-throughput sequencing and quantitative real-time PCR (qRT-PCR) for comprehensive miRNA profiling.
  • Employed Gene Ontology analysis and linear regression modeling to interpret miRNA expression data.

Main Results:

  • Identified 154 differentially expressed miRNAs (55 upregulated, 99 downregulated) between different post-mortem intervals.
  • Validated nine apoptosis-related miRNAs (five upregulated, four downregulated) using qRT-PCR.
  • Established significant correlations between the expression levels of specific miRNAs and the post-mortem interval.

Conclusions:

  • Specific miRNAs, particularly those involved in apoptosis, show promise as supportive biomarkers for PMI estimation.
  • miRNA expression patterns provide a molecular basis for improving the accuracy of forensic PMI determination.
  • This research contributes to advancing molecular methodologies in forensic science.