Pasteurization and lyophilization affect membrane proteins of pomegranate-derived nanovesicles reducing their...

Plant-derived nanovesicles (PDNVs) are promising therapeutic agents, valued for their bioactive compound content and as drug delivery systems. However, the effects of industrial food processing techniques on PDNVs remain underexplored. This study evaluated the impact of lyophilization and pasteurization on the properties and uptake efficiency of pomegranate-derived nanovesicles (PgNVs). PgNVs were isolated from pomegranate juice using tangential flow filtration and size exclusion chromatography. Treated PgNVs were analyzed via transmission electron microscopy, nanoparticle tracking analysis, and LC-MS/MS, assessing their functional properties and cellular uptake. High-purity PgNVs were obtained, although both treatments reduced their yield. Pre-treated PgNVs displayed diminished anti-inflammatory and wound-healing capacities, though antioxidant activity remained unaffected. Treated PgNVs also exhibited lower internalization by human macrophages compared to untreated PgNVs. Proteomic analyses revealed that the damage of membrane proteins, such as Tetraspanin-8, might be responsible for these effects. Our findings emphasize the need of optimizing processing to preserve PDNV therapeutic potential.