Production of bioactive peptides by high-voltage pulsed electric field: Protein extraction, mechanism, research status and collaborative application

Bioactive peptides exhibit a variety of potential applications in the fields of medicine, food and cosmetics. However, studies have shown that the traditional preparation is characterized by low efficiency, substantial pollution, limited activities and poor purity, which constrains their further application. High-voltage pulsed electric field (HPEF) technology, as a physical non-thermal processing method, shows unique advantages in bioactive peptide preparation. Through comprehensive analysis, this paper reveals the main principle of HPEF technology, the extraction of proteins (break up cellular tissue), the structural changes of proteins, enzymes and bioactive peptides after treatment, the improvement of bioactive peptides' functional properties and the potential in promoting bioactive peptides' large-scale production. Besides, this paper introduces the application of other non-thermal processing technologies, artificial intelligence and nanotechnology, providing new ways of thinking for the efficient preparation and application of bioactive peptides and establishes a theoretical foundation for the application and promotion of HPEF technology.