Related Experiment Video

Updated: May 20, 2025

Production of Human CRISPR-Engineered CAR-T Cells
06:33

Production of Human CRISPR-Engineered CAR-T Cells

Published on: March 15, 2021

12.6K

From gene editing to tumor eradication: The CRISPR revolution in cancer therapy

Ashiq Ali1, Urooj Azmat2, Aisha Khatoon3

  • 1Department of Histology and Embryology, Shantou University Medical College, Shantou, China.

Progress in Biophysics and Molecular Biology
|April 18, 2025
PubMed

Insights

CRISPR-Cas9 gene editing enhances cancer immunotherapy by modifying genes to improve treatment efficacy and reduce side effects. This technology offers new avenues for cancer treatment, research, and clinical applications.

Area of Science:

  • Oncology
  • Molecular Biology
  • Immunology

Background:

  • Cancer remains a major global health challenge with significant mortality rates.
  • Current cancer treatments, including immunotherapy, have limitations in efficacy and side effects.
  • Advancements in gene editing offer potential to improve cancer therapies.

Purpose of the Study:

  • To review CRISPR-Cas9 genome editing mechanisms and tools.
  • To analyze the impact of CRISPR-induced double-strand breaks on cancer immunotherapy.
  • To explore CRISPR-Cas9 applications in cancer research and clinical settings.

Main Methods:

  • Review of CRISPR-Cas9 technology and its application in gene knockdown and knockin.
  • Analysis of CRISPR-Cas9-based genome-wide screening for target identification.
  • Discussion of spatial CRISPR genomics and its potential.

Main Results:

  • CRISPR-Cas9 can augment cancer immunotherapy efficacy.
  • Gene editing via CRISPR-Cas9 can be used to overcome treatment limitations.
  • Genome-wide screening identifies novel therapeutic targets.

Conclusions:

  • CRISPR-Cas9 technology holds significant promise for advancing cancer immunotherapy.
  • Applications range from fundamental research to translational medicine and clinical trials.
  • Challenges in CRISPR-Cas9 implementation need to be addressed for broader clinical use.
Keywords:
CRISPR-Cas9Cancer immunotherapyDouble-strand breaks (DSBs)Gene knockdown/knockinGenome editing

More Related Videos

Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells07:56

Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells

Published on: July 22, 2019

11.1K
Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells12:04

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

Published on: March 10, 2023

3.4K

Related Concept Videos

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
48.7K
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
16.4K
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
49.9K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
7.4K
What is Genetic Engineering?00:49

What is Genetic Engineering?

Overview
73.0K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
7.3K