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RhoC GTPase Activation Assay
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YTHDF1/RNF7/p27 axis promotes prostate cancer progression
Yulin Shi1,2,3, Baiyang Liu1,2, Yong Zhang4
1The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Cell Death & Disease
|April 18, 2025
Summary
YTHDF1 protein promotes prostate cancer (PCa) growth by degrading the p27 cell cycle inhibitor via RNF7. Targeting this YTHDF1/RNF7/p27 pathway may offer new prostate cancer treatments.
Area of Science:
- Oncology
- Molecular Biology
- Epigenetics
Background:
- Prostate cancer (PCa) is a leading male cancer.
- N6-methyladenosine (m6A) modification is crucial in cancer.
- YTHDF1 is an m6A reader protein implicated in cancer.
Purpose of the Study:
- Investigate YTHDF1's role in PCa.
- Elucidate the YTHDF1/RNF7/p27 signaling axis in PCa.
- Explore therapeutic potential in PCa.
Main Methods:
- Analysis of YTHDF1 expression in PCa tissues.
- YTHDF1 knockdown in PCa cells and organoids.
- Proteasome degradation and E3 ligase screening.
- Assessment of apoptosis and drug sensitivity.
- In vitro and in vivo studies with MLN4924.
Main Results:
- YTHDF1 is highly expressed in PCa, correlating with poor outcomes.
- YTHDF1 knockdown inhibits PCa cell proliferation, migration, and tumor growth.
- YTHDF1 targets RNF7, leading to p27 degradation and promoting PCa.
- YTHDF1 or RNF7 depletion sensitizes PCa cells to cisplatin.
- MLN4924 inhibits PCa progression.
Conclusions:
- The YTHDF1/RNF7/p27 axis is vital for PCa progression.
- This axis represents a potential therapeutic target for prostate cancer.
- Targeting YTHDF1 or RNF7 may enhance chemotherapy efficacy.
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