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YTHDF1/RNF7/p27 axis promotes prostate cancer progression.

Yulin Shi1,2,3, Baiyang Liu1,2, Yong Zhang4

  • 1The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Cell Death & Disease
|April 18, 2025
PubMed
Summary
This summary is machine-generated.

YTHDF1 protein promotes prostate cancer (PCa) growth by degrading the p27 cell cycle inhibitor via RNF7. Targeting this YTHDF1/RNF7/p27 pathway may offer new prostate cancer treatments.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • Prostate cancer (PCa) is a leading male cancer.
  • N6-methyladenosine (m6A) modification is crucial in cancer.
  • YTHDF1 is an m6A reader protein implicated in cancer.

Purpose of the Study:

  • Investigate YTHDF1's role in PCa.
  • Elucidate the YTHDF1/RNF7/p27 signaling axis in PCa.
  • Explore therapeutic potential in PCa.

Main Methods:

  • Analysis of YTHDF1 expression in PCa tissues.
  • YTHDF1 knockdown in PCa cells and organoids.
  • Proteasome degradation and E3 ligase screening.
  • Assessment of apoptosis and drug sensitivity.
  • In vitro and in vivo studies with MLN4924.

Main Results:

  • YTHDF1 is highly expressed in PCa, correlating with poor outcomes.
  • YTHDF1 knockdown inhibits PCa cell proliferation, migration, and tumor growth.
  • YTHDF1 targets RNF7, leading to p27 degradation and promoting PCa.
  • YTHDF1 or RNF7 depletion sensitizes PCa cells to cisplatin.
  • MLN4924 inhibits PCa progression.

Conclusions:

  • The YTHDF1/RNF7/p27 axis is vital for PCa progression.
  • This axis represents a potential therapeutic target for prostate cancer.
  • Targeting YTHDF1 or RNF7 may enhance chemotherapy efficacy.