Transcriptome Analysis Reveals Possible Antitumor Mechanism of Intracellular Polysaccharide From Phaeodactylum tricornutum on Cervical Cancer HeLa Cells
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View abstract on PubMed
Summary
This summary is machine-generated.Seaweed polysaccharides from Phaeodactylum tricornutum show potent antitumor activity against cervical cancer cells. The fraction PRP4 effectively inhibited cancer cell proliferation and viability, indicating therapeutic potential.
Area Of Science
- Marine Biotechnology
- Cancer Research
- Natural Products Chemistry
Background
- Seaweed polysaccharides are recognized for their non-toxic antitumor properties.
- Intracellular polysaccharides from Phaeodactylum tricornutum (PRP) are being investigated for therapeutic applications.
Purpose Of The Study
- To isolate and purify PRP fractions from Phaeodactylum tricornutum.
- To evaluate the antitumor effects of these fractions on cervical cancer cells.
- To elucidate the underlying molecular mechanisms of action.
Main Methods
- Isolation and purification of seaweed polysaccharide fractions.
- In vitro evaluation of anti-cervical cancer cell activity using CCK-8 assays.
- Transcriptome analysis to identify differentially expressed genes (DEGs).
- Gene function enrichment analysis and protein-protein interaction (PPI) network construction.
Main Results
- All four purified PRP fractions inhibited HeLa cell proliferation, viability, and altered cell morphology.
- PRP4 demonstrated the most significant inhibitory effect.
- Transcriptome analysis revealed 806 DEGs, with enrichment in apoptosis and tumor-related pathways.
- Ten hub genes, including TLR4, IL1B, and HMOX1, were identified via PPI network analysis.
Conclusions
- PRP fractions, particularly PRP4, exhibit significant antitumor potential against cervical cancer.
- The antitumor effects are mediated through modulation of key cancer and apoptosis signaling pathways.
- PRP4 warrants further investigation as a potential therapeutic agent for cancer treatment.
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