Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Simple stability-indicating assay for histamine solutions.

R K Marwaha, B F Johnson, G E Wright

    American Journal of Hospital Pharmacy
    |July 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Assessment of anteroposterior instability of the knee during gait.

    Journal of the Japanese Physical Therapy Association = Rigaku ryoho·2015
    Same author

    Modified diadenosine tetraphosphates with dual specificity for P2Y1 and P2Y12 are potent antagonists of ADP-induced platelet activation.

    Journal of thrombosis and haemostasis : JTH·2012
    Same author

    Bioavailability of digoxin from rapidly dissolving preparations.

    British journal of clinical pharmacology·2012
    Same author

    Psychiatric genetics in South Africa: cutting a rough diamond.

    African journal of psychiatry·2011
    Same author

    Childhood acute lymphoblastic leukemia: need of a national population based registry.

    Indian pediatrics·2011
    Same author

    Imatinib has adverse effect on growth in children with chronic myeloid leukemia.

    Pediatric blood & cancer·2011
    Same journal

    National health care reform, Part 2: Response to pharmacists.

    American journal of hospital pharmacy·1994
    Same journal

    Validity of originality assessment.

    American journal of hospital pharmacy·1994
    Same journal

    Unit dose dispensing of chromic phosphate P 32 suspension.

    American journal of hospital pharmacy·1994
    Same journal

    Hydralazine injection still available.

    American journal of hospital pharmacy·1994
    Same journal

    Improved extemporaneous formulation of cyclosporine ophthalmic drops.

    American journal of hospital pharmacy·1994
    Same journal

    Paclitaxel diluent and the case of the slippery spike.

    American journal of hospital pharmacy·1994
    See all related articles

    A new colorimetric assay using the Pauly reaction accurately measures histamine stability in buffer solutions. This method is more reliable than the Folin reaction assay, especially under UV light exposure.

    Area of Science:

    • Analytical Chemistry
    • Biochemistry

    Background:

    • Histamine analysis is crucial for pharmaceutical and biological research.
    • Existing analytical methods may lack specificity or be susceptible to degradation products.
    • Stability-indicating assays are essential for accurate histamine quantification.

    Purpose of the Study:

    • To develop and evaluate a chemical stability-indicating assay for histamine in isotonic-phosphate buffer solutions.
    • To compare the suitability of the Pauly reaction (imidazole group) versus the Folin reaction (amino group) for histamine analysis.
    • To assess the impact of environmental factors like UV irradiation and heat on histamine assay accuracy.

    Main Methods:

    • Developed a quantitative colorimetric assay adapting the Pauly reaction for histamine determination.

    Related Experiment Videos

  • Utilized histamine diphosphate standards for calibration.
  • Evaluated assay specificity by subjecting samples to UV irradiation and heat.
  • Compared results with the USP assay method based on the Folin reaction.

    • Main Results:

    • Both Pauly and Folin assays produced reproducible linear calibration curves.
    • The Pauly-reaction assay demonstrated high precision (CVs 0.5% between, 0.1% within runs) and recovery (100.6-100.8%).
    • UV irradiation significantly decreased apparent histamine concentrations (up to 69.2%) with the Pauly assay, while heat caused minimal degradation (6.3%).
    • UV irradiation increased apparent histamine concentrations (up to 50.6%) with the Folin assay.

    Conclusions:

    • The Pauly colorimetric assay, targeting the imidazole group, is more appropriate for histamine solutions than the Folin-reaction assay.
    • Histamine stability is compromised by UV irradiation, affecting assay accuracy.
    • The developed Pauly-based assay is a reliable method for routine histamine analysis in buffer solutions.