Synthetic Engineering of Microbes for Production of Terpenoid Food Ingredients

Terpenoids are a class of chemicals comprising many food ingredient chemicals. Synthetic biology and metabolic engineering have been performed to produce microbial cell factories for their production. For improved production of various terpenoid ingredients, heterologous synthetic pathways can be optimized at multiple dimensions. Optimizing chassis precursor supply and overcoming the host's inherent metabolic rigidity are crucial for enhancing overall efficiency of heterologous terpenoid production. Integrating synthetic regulatory circuits can facilitate the staged programming and precise optimization of heterologous and endogenous metabolism. Engineering long-term genetic and metabolic stability is essential for the successful scale-up of commercial production. Maximizing efficiency in food terpenoid production will rely on interdisciplinary synthetic and engineering biology tools to advance state-of-the-art capabilities for the streamlined design and construction of complex genotypes in microbial chassis.