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    Area of Science:

    • Structural Biology
    • Biophysics
    • Microscopy

    Background:

    • Cryo-electron microscopy (cryo-EM) has revolutionized structural biology.
    • It enables high-resolution imaging of biological macromolecules.

    Purpose of the Study:

    • To provide an overview of cryo-electron microscopy techniques.
    • To discuss current challenges in the field of cryo-EM.

    Main Methods:

    • Single particle analysis (SPA)
    • Cryo-electron tomography (cryo-ET)
    • Microcrystal electron diffraction (MicroED)

    Main Results:

    • Cryo-EM encompasses diverse and complementary methodologies.
    • Each technique offers unique advantages for structural determination.

    Conclusions:

    • Cryo-EM continues to be a pivotal technology in structural biology.
    • Addressing current challenges will further expand its applications.