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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

241
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
241

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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
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White Light Transmission Spectroscopy for Rapid Quality Control Imperfection Identification in Nanoimprinted

Mike Hardy1, Hin On Martin Chu2,3, Serene Pauly1,4

  • 1Smart Nano NI, Centre for Quantum Materials and Technologies, School of Mathematics and Physics, Queen's University Belfast, Belfast BT7 1NN, United Kingdom.

ACS Measurement Science Au
|April 21, 2025
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Summary

This study investigates nanoimprint lithography (NIL) for biomedical sensors, finding principal component analysis (PCA) effectively detects substrate angle imperfections. Gold and iron nanodomes show promise for surface-enhanced Raman spectroscopy (SERS) applications.

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Spectroscopy

Background:

  • Miniaturized biomedical sensors require cost-effective and scalable fabrication methods.
  • Nanoimprint lithography (NIL) offers promise but faces challenges in pattern reproducibility.
  • High-fidelity nanostructures are crucial for advanced sensing applications like SERS and SPR.

Purpose of the Study:

  • To investigate the quality control of NIL-produced nanostructures for sensing.
  • To assess the impact of substrate imperfections on optical and spectroscopic measurements.
  • To evaluate the performance of gold- and iron-coated nanodomes for SERS applications.

Main Methods:

  • Utilized white light transmission spectroscopy and principal component analysis (PCA) to analyze polyurethane nanodome arrays.
  • Investigated plasmon polariton interactions using a coupled two-oscillator model.
  • Performed surface-enhanced Raman spectroscopy (SERS) on gold- and iron-coated nanodomes.
  • Employed scanning thermal probe microscopy to characterize thermal properties of nanostructures.

Main Results:

  • PCA effectively identified large angle mismatches (2-10°) in NIL substrates with statistical significance (p=0.05).
  • Observed spectral dips potentially due to interacting localized and propagating plasmon polaritons.
  • Gold-coated nanodomes achieved 6-10% relative standard deviation in SERS measurements of gelatin.
  • Iron-coated nanodomes exhibited useful SERS enhancement and significant local temperature increases (up to 5 °C).

Conclusions:

  • Developed a framework for rapid quality control of NIL nanostructures using PCA.
  • Demonstrated the potential of NIL for fabricating nanostructures suitable for SERS and SPR sensing.
  • Highlighted the thermal stability of nanostructures even under high local temperatures.
  • Provided insights into interpreting PCA loadings for physical phenomena in nanostructure analysis.