Physiological and biochemical response of mixed lupine and barley cultures under changing environmental conditions...

Mixed cultivation of grass-legume forage crops, such as lupine (Lupinus albus L.) and barley (Hordeum vulgare L.), offers significant advantages in terms of nitrogen utilization, stress resistance and a balanced diet for ruminants. This study explored the symbiotic effects of these crops on photosynthesis and stress tolerance via measuring key physiological and biochemical parameters. Measurements were performed on the photosynthetic activity, chlorophyll and carotenoid content, glycolate oxidase activity, antioxidant capacity, and total phenolic content. The varying temperatures during May, allowed the effects of mixed cultivation on the response to chilling to be analyzed. Notably, barley monoculture was the most affected by the decreased temperatures. In general, mixed culture showed mitigation of the effects from chilling, as compared with both lupine and barley monocultures alone. These results suggest an adaptive synergy between lupine and barley, highlighting the potential advantages of mixed cultivation for improving stress tolerance and overall crop performance.