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Updated: May 10, 2025

Production and Detection of Reactive Oxygen Species ROS in Cancers
07:17

Production and Detection of Reactive Oxygen Species ROS in Cancers

Published on: November 21, 2011

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Reactive oxygen species (ROS) are a crucial factor in the anticancer activity of Oliveria decumbens extract against

M Dinarvand1, F Sharifnia1, Z Jangravi2

  • 1Department of biology, North Tehran Branch, Islamic Azad University, Tehran, Iran.

Archives of Razi Institute
|April 21, 2025
PubMed

Insights

Oliveria decumbens extract shows potential as a natural skin cancer therapy. It inhibits cancer cell proliferation by inducing cell cycle arrest, apoptosis, and increasing reactive oxygen species (ROS).

Area of Science:

  • Phytochemistry
  • Oncology
  • Natural Product Research

Background:

  • Skin cancer incidence is rising globally, necessitating novel therapeutic strategies.
  • Phytochemicals offer cost-effective, low-side-effect alternatives for cancer treatment development.
  • Limitations of conventional therapies drive research into plant-derived compounds.

Purpose of the Study:

  • To investigate the anti-cancer effects of Oliveria decumbens (O. decumbens) aqueous extract on human skin cancer A431 cells.
  • To determine the extract's impact on cell viability, apoptosis, cell cycle progression, and reactive oxygen species (ROS) production.
  • To evaluate the potential of O. decumbens as a future skin cancer treatment option.

Main Methods:

  • Preparation of O. decumbens aqueous extract using a traditional method.
  • Assessment of cell viability and determination of IC50 using the MTT assay.
  • Analysis of apoptosis via Annexin V-FITC/Propidium Iodide staining and flow cytometry.
  • Investigation of cell cycle distribution using Propidium Iodide staining and flow cytometry.
  • Quantification of reactive oxygen species (ROS) production using DCFH-DA staining and flow cytometry.

Main Results:

  • The IC50 for cell viability was determined to be 475 µg/ml.
  • O. decumbens extract induced G1 phase arrest in the cell cycle of treated A431 cells.
  • Apoptosis significantly increased in treated cells (8.2%) compared to controls (2.5%).
  • A significant increase in ROS levels was observed in O. decumbens treated cells (738) versus controls (316).

Conclusions:

  • O. decumbens extract demonstrates significant anti-cancer activity against human skin cancer cells.
  • The extract inhibits cancer cell proliferation by inducing cell cycle arrest and apoptosis.
  • Increased ROS production appears to play a critical role in the anti-cancer effects of O. decumbens.
  • O. decumbens holds potential as a therapeutic option for future skin cancer treatment.
Keywords:
A431cell lineAqueous extractOliveriadecumbensROSSkin cancer

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