A field method for the procurement of infective larvae of Onchocerca volvulus

Natural vectors of onchocerciasis (S. yahense) were collected. Ninety-eight were intrathoracically inoculated with 30 freshly obtained skin-dwelling microfilariae. Of the 82 flies dissected, 56 were infected and 28 harbored infective larvae. A total of 193 infective O. volvulus larvae were obtained. The mean infective worm burden was 6.9.