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Updated: May 10, 2025

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP
Published on: September 8, 2023
Reverse-Transcriptase Loop-Mediated Isothermal Amplification (RT-LAMP) for Viral Detection - A Case Study
Ashley Hassman1, Renée Richie Casey1, Colby Rouchka1
1College of Health Solutions, Arizona State University, Tempe, Arizona.
Abstract:
An assay and protocol framework are provided for utilizing reverse-transcriptase loop-mediated isothermal amplification (RT-LAMP) at the point-of-care for diagnosing and monitoring a hypothetical zoonotic viral outbreak in a resource-limited area. This manuscript utilizes a previously published decision tree algorithm to determine an appropriate molecular diagnostic point-of-care test that can effectively address the outbreak presented in the hypothetical case study. © 2025 Wiley Periodicals LLC.
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