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Transcriptional Regulation Mechanisms in AsAFL1-mediated Drought Tolerance for Creeping Bentgrass (Agrostis
Zhenzhen Tan1,2, Yiting Wang1, Yu Liu3
1College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing, China.
Abstract:
Drought stress is a major environmental stress that impairs plant growth and development. The At14a-like1 (AFL1) gene encodes a stress-induced membrane protein involved in endocytosis, signal transduction, and proline accumulation. The objective of the present study was to investigate biological functions and underlying mechanisms of AFL1 regulation of drought tolerance in a perennial grass species, creeping bentgrass (Agrostis stolonifera). AsAFL1 was cloned from creeping bentgrass, and its expression was induced by drought stress. Motif analysis showed that AsAFL1 has five epidermal growth factor structural domains and one β1-integrin structural domain. Transient expression in tobacco epidermal cells indicated that AsAFL1 was localized at the plasma membrane. Overexpression of AsAFL1 in creeping bentgrass significantly enhanced drought tolerance, as manifested by significantly increased leaf relative water content, chlorophyll and proline contents but lower electrolyte leakage and malondialdehyde content. Comparative transcriptomic and weighted correlation network analysis (WGCNA) revealed that AsAFL1-mediated drought tolerance was related to transcriptional regulation of genes involved in phytohormone (abscisic acid, auxin, and strigolactone) biosynthesis and signaling, redox homeostasis, and biosynthesis of second metabolites (lignin, cutin, suberin and wax), as well as nutrient transport and mobilization.
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Transcription
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
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Regulation of Transpiration by Stomata
Responses to Salt Stress
Transcription Attenuation in Prokaryotes
There are several different mechanisms used to attenuate transcription. In ribosome mediated...