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Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
Published on: September 15, 2017
Natural anti-neuroinflammatory inhibitors from Aglaia odorata
Xin-Yang Liu1, Jing-Jing Zhang1, Qian-Qian Yin1
1School of Traditional Chinese Materia Medica, Key Laboratory of Innovative Traditional Chinese Medicine for Major Chronic Diseases of Liaoning province, Key Laboratory for TCM Material Basis Study and Innovative Drug Development of Shenyang City, Shenyang Pharmaceutical University, Shenyang 110016, China.
Abstract:
Eleven compounds were isolated from the n-butanol extract of Aglaia odorata Lour, including one undescribed lignan, curlignan-4-O-β-D-glucopyranoside (1), and ten known compounds (2-11). Their structures were characterized by comprehensive spectral analysis, nuclear magnetic resonance (1D and 2D NMR), mass spectrometry (MS), and electronic circular dichroism (ECD). The inhibitory effects of these compounds on nitric oxide (NO) production in lipopolysaccharide (LPS) stimulated BV-2 microglia were evaluated. The experimental results indicated that the anti-neuroinflammatory activities of compounds 1, 3, 4, 6, 7, 9 and 10 were significantly better than those of the positive control minocycline (IC50 = 41.34 ± 3.55 μM), with IC50 values ranging from 1.31 to 15.70 μM.
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