Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Patch Clamp01:18

Patch Clamp

5.3K
Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...
5.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

O-linked glycan-dependent gating of TPC2 controls lysosomal excitability and organelle remodeling.

Nature communications·2026
Same author

Comparative Analysis of Temperature-Dependent Endocytic Regulation of Notch Signaling in Chick and Mouse Neural Progenitors.

Cells, tissues, organs·2026
Same author

Genomic and transcriptomic characterization of acute myeloid leukaemia with IL3RA overexpression: Prognostic and therapeutic implications revisited.

British journal of haematology·2025
Same author

TRPML2 channel modulation by PI(3,5)P₂ and small-molecule agonists controls endosomal vesicle dynamics.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2025
Same author

C1GALT1 expression predicts poor survival in osteosarcoma and is crucial for ABCC1 transporter-mediated doxorubicin resistance.

The Journal of pathology·2025
Same author

Rab7a is an enhancer of TPC2 activity regulating melanoma progression through modulation of the GSK3β/β-Catenin/MITF-axis.

Nature communications·2024
Same journal

A Video Protocol of a Randomized Controlled Clinical Trial - Electrochemotherapy of Cutaneous Metastases with Reduced Dose Bleomycin (BLESS Trial).

Journal of visualized experiments : JoVE·2026
Same journal

A Standardized Ex Vivo Porcine Oromucosal Model for Evaluating Peptide Fluxes.

Journal of visualized experiments : JoVE·2026
Same journal

Lightweight English Text Classification with Deep Learning Based on Complex System Theory.

Journal of visualized experiments : JoVE·2026
Same journal

Integrating Artificial Intelligence-Assisted Translation Support into English Courses: Effects on Translation Accuracy, Perceived Stress, and Anxiety.

Journal of visualized experiments : JoVE·2026
Same journal

A Toxin-Based Counter-Selection System for Markerless Gene Deletion and High-Density Tn5 Transposon Mutagenesis in Pectobacterium brasiliense.

Journal of visualized experiments : JoVE·2026
Same journal

Seamless Multimodal Human-Robot Communication: Integration Techniques in Human-Computer Interaction.

Journal of visualized experiments : JoVE·2026
See all related articles

Related Experiment Video

Updated: May 10, 2025

Patch-Clamp Techniques for Single Endolysosomal Vesicle Analysis
05:47

Patch-Clamp Techniques for Single Endolysosomal Vesicle Analysis

Published on: April 4, 2025

121

Patch-Clamp Techniques for Single Endolysosomal Vesicle Analysis.

Hsuan-Ti Wang1, Cheng-Chang Chen2

  • 1Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University.

Journal of Visualized Experiments : Jove
|April 21, 2025
PubMed
Summary
This summary is machine-generated.

Endolysosomal patch-clamp techniques allow direct measurement of ion channel activity in endolysosomes. This method aids in discovering new drug targets for diseases linked to ion channel dysfunction.

More Related Videos

F1FO ATPase Vesicle Preparation and Technique for Performing Patch Clamp Recordings of Submitochondrial Vesicle Membranes
08:21

F1FO ATPase Vesicle Preparation and Technique for Performing Patch Clamp Recordings of Submitochondrial Vesicle Membranes

Published on: May 4, 2013

10.2K
Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology
09:03

Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology

Published on: June 21, 2013

22.1K

Related Experiment Videos

Last Updated: May 10, 2025

Patch-Clamp Techniques for Single Endolysosomal Vesicle Analysis
05:47

Patch-Clamp Techniques for Single Endolysosomal Vesicle Analysis

Published on: April 4, 2025

121
F1FO ATPase Vesicle Preparation and Technique for Performing Patch Clamp Recordings of Submitochondrial Vesicle Membranes
08:21

F1FO ATPase Vesicle Preparation and Technique for Performing Patch Clamp Recordings of Submitochondrial Vesicle Membranes

Published on: May 4, 2013

10.2K
Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology
09:03

Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology

Published on: June 21, 2013

22.1K

Area of Science:

  • Cell Biology
  • Biophysics
  • Physiology

Background:

  • Endolysosomal ion channels regulate crucial cellular processes like pH homeostasis and vesicle transport.
  • Electrophysiological access to these channels in small vesicles has been a significant technical challenge.
  • Dysfunctional endolysosomal ion channels are implicated in various diseases, including neurodegenerative and metabolic disorders.

Purpose of the Study:

  • To introduce and validate endolysosomal patch-clamp techniques for studying ion channel activity.
  • To highlight the advantages of endolysosomal patch-clamp over existing methods.
  • To underscore the potential of targeting endolysosomal ion channels for therapeutic interventions.

Main Methods:

  • Development and application of endolysosomal patch-clamp techniques.
  • Direct electrophysiological recording of ion channel activity in endolysosomal membranes.
  • Comparison with conventional planar patch-clamp methods.

Main Results:

  • Endolysosomal patch-clamp enables direct measurement of ion channel activity in endolysosomes.
  • This technique offers advantages such as multi-cell recording, compatibility with other methods, and enhanced experimental flexibility (e.g., Ca2+ manipulation).
  • Manual operation allows for precise visualization and targeting of vesicles.

Conclusions:

  • Endolysosomal patch-clamp is a powerful tool for investigating ion channel function within endolysosomes.
  • Targeting endolysosomal ion channels presents a promising strategy for developing treatments for associated diseases.
  • This technique is pivotal for drug discovery and the development of novel therapeutics aimed at restoring intracellular ion balance.