Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Nitrogen Metabolism01:20

Overview of Nitrogen Metabolism

7.7K
Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of  ammonia, ammonium ions, nitrate, nitrite, or  nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
7.7K
Bioremediation00:46

Bioremediation

18.1K
Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.
18.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessing contribution of anaerobic methane oxidation and its active methanotrophic communities in landfills.

Waste management (New York, N.Y.)·2026
Same author

Role of hydrochar in enhancing nitrous oxide sink in microaerobic sequencing batch reactors.

Bioresource technology·2026
Same author

A review of metallurgical processing and value-added utilization strategies for zinc oxide.

Science progress·2026
Same author

Continuous leachate irrigation as an ammonia source enhancing methane oxidation in landfill biocover soils.

Journal of environmental management·2026
Same author

Nitrate amendment improving the stability of methane oxidation in landfill biocover soils.

Environmental research·2025
Same author

Fine hydrochar in hydrothermal hydrolysate promotes startup and gas production of swine manure anaerobic digestion.

Scientific reports·2025

Related Experiment Video

Updated: May 10, 2025

Author Spotlight: Advancing Anaerobic Microbiota Research Using a Novel Respirometry Protocol
06:11

Author Spotlight: Advancing Anaerobic Microbiota Research Using a Novel Respirometry Protocol

Published on: April 26, 2024

1.1K

Methane enhancing nitrous oxide consumption in microaerobic sludge systems.

Ruo He1, Xinyue Li1, Xin Zhang1

  • 1Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, 310012, China.

Environmental Research
|April 21, 2025
PubMed
Summary

Microaerobic sludge systems efficiently consume nitrous oxide (N₂O) and methane (CH₄) simultaneously. Higher oxygen levels and methane presence significantly enhance N₂O reduction, suggesting a coupled process for emission mitigation.

Keywords:
Greenhouse gasesMetagenomic sequencingMethane oxidationMicrobial synergyNitrous oxide consumption

More Related Videos

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
06:17

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions

Published on: August 15, 2019

27.4K
Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
09:38

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

8.5K

Related Experiment Videos

Last Updated: May 10, 2025

Author Spotlight: Advancing Anaerobic Microbiota Research Using a Novel Respirometry Protocol
06:11

Author Spotlight: Advancing Anaerobic Microbiota Research Using a Novel Respirometry Protocol

Published on: April 26, 2024

1.1K
Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
06:17

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions

Published on: August 15, 2019

27.4K
Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
09:38

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

8.5K

Area of Science:

  • Environmental Microbiology
  • Biogeochemical Cycles
  • Wastewater Treatment

Background:

  • Nitrous oxide (N₂O) reduction is typically associated with anoxic or hypoxic conditions.
  • Methane (CH₄) oxidation is a key process in mitigating greenhouse gas emissions.
  • Understanding simultaneous CH₄ oxidation and N₂O reduction in microaerobic environments is crucial for effective emission control.

Purpose of the Study:

  • To investigate the simultaneous oxidation of methane (CH₄) and consumption of nitrous oxide (N₂O) in microaerobic sludge systems.
  • To compare the performance of these processes under different dissolved oxygen (DO) concentrations and in the presence/absence of CH₄.
  • To identify microbial communities involved in CH₄ oxidation and N₂O reduction under microaerobic conditions.

Main Methods:

  • Experimental setup using microaerobic sludge systems with controlled dissolved oxygen (DO) levels (∼38.2 and ∼12.1 µM).
  • Monitoring of CH₄ oxidation and N₂O consumption rates under varying conditions, including the presence of CH₄.
  • Microbial community analysis to identify methane-oxidizing bacteria (MOB) and denitrifying bacteria.

Main Results:

  • Higher dissolved oxygen (DO) concentrations in sludge systems with CH₄ showed significantly higher N₂O consumption rates (3.4-4.9 times greater).
  • A positive linear correlation was observed between CH₄ oxidation rate and N₂O consumption rate.
  • Type I MOB (e.g., Methylococcus) were more abundant than Type II MOB, and Anaerolinea and Lautropia were key denitrifying bacteria.

Conclusions:

  • Simultaneous CH₄ oxidation and N₂O consumption occur effectively in microaerobic sludge systems.
  • Appropriate oxygen concentrations, coupled with CH₄ presence, enhance N₂O reduction, indicating a potentially linked microbial process.
  • These findings suggest microaerobic systems can be optimized for simultaneous mitigation of CH₄ and N₂O emissions.