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Related Experiment Video

Updated: May 1, 2026

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

A streamlined POCT solution for rapid infectious disease detection.

Yaping Xie¹, Yan Yu¹, Shuaiwu Huang¹

¹Research and Development Department, Sansure Biotech Inc., Changsha, 410125, China.

Scientific Reports

|April 21, 2025

Summary

A new integrated point-of-care testing (POCT) system, iPonatic, provides rapid infectious disease results in 30 minutes. This advanced POCT enhances diagnostics and reduces patient wait times for public health needs.

Keywords:

One-step nucleic acid release technology Point-of-care Quantitative dropper Respiratory infection Sexually transmitted infection iPonatic

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Area of Science:

Biotechnology
Medical Diagnostics
Infectious Disease Research

Background:

Point-of-care testing (POCT) is vital for infectious disease screening, but current systems suffer from long preparation times and complex procedures.
Challenges include lengthy sample preparation, difficult nucleic acid extraction, and limited real-time data, leading to extended patient waiting periods.

Purpose of the Study:

To introduce iPonatic, an integrated POCT system designed to overcome the limitations of existing technologies.
To enable rapid, efficient, and real-time infectious disease diagnostics at the point of care.

Main Methods:

Development of iPonatic featuring a quantitative dropper, a low-cost cartridge with one-step nucleic acid release, and cloud-based real-time data synchronization.
Validation of the system for respiratory and sexually transmitted pathogens using quantitative PCR (qPCR) as a benchmark.
Clinical validation involving 1159 samples to assess diagnostic performance.

Main Results:

The iPonatic system demonstrated consistent [Formula: see text] values compared to standard qPCR.
Clinical validation showed an Area Under the Curve (AUC) exceeding 0.98 for all tested targets.
Achieved high diagnostic accuracy with sensitivity over 95% and specificity of 100%.

Conclusions:

iPonatic offers a significant advancement in POCT for infectious diseases, delivering results within 30 minutes.
The system is well-suited for primary healthcare, epidemic response, and resource-limited settings.
iPonatic enhances diagnostic efficiency, reduces patient wait times, and supports timely public health interventions.