Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

9.1K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.1K
General Transcription Factors01:30

General Transcription Factors

5.2K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.2K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

875
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
875
Condensins02:15

Condensins

3.4K
Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
3.4K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

22.5K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.5K
Transcription Factors02:16

Transcription Factors

75.7K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
75.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Association of postoperative delirium with haemodynamic determinants of cerebral perfusion pressure during cardiac surgery: a retrospective cohort study.

British journal of anaesthesia·2026
Same author

BMI1 regulates human erythroid self-renewal through both gene repression and gene activation.

Nature communications·2025
Same author

Histone H4 lysine 20 methylation marks genes dynamically regulated during erythroid maturation.

Epigenetics & chromatin·2025
Same author

Fine-Mapping the Association of Acute Kidney Injury With Mean Arterial and Central Venous Pressures During Coronary Artery Bypass Surgery.

Anesthesia and analgesia·2025
Same author

Identification of small molecule inhibitors of PPM1D using an integrated drug discovery platform.

iScience·2025
Same author

Correction: Performance evaluation of a combination Plasmodium dual-antigen CRP rapid diagnostic test in Lambaréné, Gabon.

Infection·2024

Related Experiment Video

Updated: Jun 13, 2025

Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA
07:05

Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA

Published on: September 8, 2021

2.4K

The Condensin II complex regulates essential gene expression programs during erythropoiesis.

Deanna Abid1, Kristin Murphy1, Zachary Murphy1,2

  • 1Department of Pediatrics, University of Rochester Medical Center, Rochester, NY 14642, USA.

Development (Cambridge, England)
|April 22, 2025
PubMed
Summary
This summary is machine-generated.

The study reveals that NCAPH2, a subunit of Condensin II, is essential for red blood cell development (erythropoiesis). Its loss causes severe anemia and disrupts gene expression critical for cell maturation and proliferation.

Keywords:
Cell cycleCondensin II ComplexEpigeneticErythropoiesisH4K20me1Mouse

More Related Videos

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

9.7K
Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
15:32

Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay

Published on: August 5, 2011

32.3K

Related Experiment Videos

Last Updated: Jun 13, 2025

Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA
07:05

Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA

Published on: September 8, 2021

2.4K
Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

9.7K
Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
15:32

Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay

Published on: August 5, 2011

32.3K

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Genetics

Background:

  • Erythropoiesis involves significant gene expression changes and nuclear condensation.
  • Mechanisms maintaining erythroid gene expression and nuclear condensation are not fully understood.
  • Condensin II complex regulates chromatin condensation and gene expression.

Purpose of the Study:

  • To investigate the role of Condensin II, specifically the NCAPH2 subunit, in erythropoiesis.
  • To understand how NCAPH2 influences gene expression and nuclear condensation during red blood cell development.

Main Methods:

  • Generated an erythroid-specific deletion of the Ncaph2 gene in mice.
  • Analyzed erythroid cell maturation, cell cycle progression, and nuclear morphology.
  • Performed genomic studies to identify NCAPH2-occupied gene promoters.

Main Results:

  • Ncaph2 loss led to severe anemia and embryonic lethality by day 12.5.
  • Mutant erythroid cells exhibited dysregulated maturation and cell cycle defects.
  • NCAPH2 was found to be dispensable for nuclear condensation but occupied promoters of downregulated erythroid and cell cycle genes.

Conclusions:

  • NCAPH2 plays a crucial role in regulating gene expression programs essential for erythroid differentiation and cell cycle progression.
  • The Condensin II complex is involved in controlling lineage-specific differentiation programs.