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Updated: May 10, 2025

Application of Two-spotted Spider Mite Tetranychus urticae for Plant-pest Interaction Studies
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Evaluating broad mite (Acari: Tarsonemidae) sampling techniques in blackberry.

Jared B Linn1, Aaron J Cato2, Amanda L McWhirt2

  • 1Department of Entomology and Plant Pathology, University of Arkansas, Fayetteville, AR, USA.

Journal of Economic Entomology
|April 22, 2025
PubMed
Summary

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A new scouting method for broad mite (Polyphagotarsonemus latus) in blackberry fields uses visual injury assessments. This method is more practical for growers than leaf sampling, improving pest management strategies.

Area of Science:

  • Agricultural Entomology
  • Plant Pathology
  • Pest Management

Background:

  • Broad mite (Polyphagotarsonemus latus) is a significant pest affecting global blackberry production.
  • Current recommendations for miticide application are based on small plot studies, lacking field-scale validation.
  • Growers often rely on visual injury assessments, but optimal scouting methods for broad mites at the field scale are uninvestigated.

Purpose of the Study:

  • To identify an effective field-scale scouting strategy for estimating broad mite density.
  • To investigate the relationship between broad mite density and visual injury symptoms.
  • To develop a practical visual injury sampling strategy for broad mite management in blackberry.

Main Methods:

  • Evaluated three leaflet sample sizes (5, 10, 15 leaflets) across 12 commercial blackberry fields.
Keywords:
detection samplinghorticulture entomologyintegrated pest management

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  • Assessed visual injury using a descriptive scale (1-5) in 33 commercial fields.
  • Employed regression analysis to correlate mite density with visual injury and determine optimal sample sizes.

    • Main Results:

    • Fifteen-leaflet samples yielded the best estimate of mite populations, indicated by the lowest variance-to-mean ratio (12.2) for motile mites.
    • Estimating mite density at threshold with 70% accuracy required 31 samples of 15 leaflets.
    • A positive correlation was found between mite density and visual injury; a rating of two indicated 72% of samples exceeded the threshold.

    Conclusions:

    • Visual injury assessment can effectively estimate broad mite populations in blackberry fields.
    • A sampling strategy based on visual injury (11 replicates, 10-cane samples) is more feasible for growers than labor-intensive leaf sampling.
    • The findings support the adoption of visual injury scouting for improved broad mite management by growers.