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Updated: May 10, 2025
Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
Published on: August 25, 2019
Antinuclear antibodies do not affect euploidy rate in preimplantation genetic testing for aneuploidy
Mei Liu1, Yueyue Hu1, Honglu Diao2
1Reproductive Medicine Centre, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei, P.R. China; Hubei Clinical Research Centre for Reproductive Medicine, Shiyan, Hubei, P.R. China; Biomedical Engineering College, Hubei University of Medicine, Shiyan, Hubei, P.R. China.
Research Question:
Do antinuclear antibodies affect the blastocyst aneuploidy rate during preimplantation genetic testing for aneuploidy (PGT-A) cycles?
Design:
A retrospective cohort study was undertaken of 259 patients who underwent PGT-A and immunological screening at the study centre between May 2018 and May 2023. Patients were divided into an antinuclear-antibody-negative group (213 cycles) and an antinuclear-antibody-positive group (46 cycles) based on the presence/absence of antinuclear antibodies, and embryo outcomes were compared.
Results:
Significant differences were found between antinuclear-antibody-negative and antinuclear-antibody-positive patients in terms of paternal age (34 versus 37 years; P = 0.032), maternal age (33 versus 35 years; P = 0.046) and duration of infertility (2 versus 3 years; P = 0.009). No significant differences were found in the number of retrieved oocytes, metaphase II (MII) oocytes, available embryos, high-quality embryos, blastocysts biopsied, euploid blastocysts, MII oocyte rate, fertilization rate, high-quality embryo rate and euploidy rate. Multiple linear regression revealed that, in infertile couples who underwent PGT-A, maternal age was an independent factor influencing the euploidy rate (regression coefficient -0.009), whereas the presence of antinuclear antibodies was not an independent factor influencing the euploidy rate.
Conclusion:
In couples who underwent PGT-A, the presence of antinuclear antibodies did not affect the euploidy rate. This study did not observe any negative effects of antinuclear antibodies on oocyte or embryo outcomes.
