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[Immunochemical study of blastolysin].
Antibiotiki I Meditsinskaia Biotekhnologiia = Antibiotics and Medical Biotechnology
|April 1, 1985
Summary
Blastolysin (BL), derived from Lactobacillus bulgaricus cell walls, shows low immunogenicity but induces specific antibodies in animals. Immunochemical methods are crucial for standardizing this potential pharmacological agent.
Area of Science:
- Immunology
- Microbiology
- Pharmacology
Context:
- Blastolysin (BL) is a preparation from Lactobacillus bulgaricus cell walls.
- BL exhibits immunostimulating and antitumor activities.
- Understanding BL's immunochemical properties is essential for its therapeutic application.
Purpose:
- To investigate the immunochemical properties of blastolysin (BL).
- To assess the immunogenicity and antibody production following BL administration.
- To explore the potential of immunochemical procedures for BL standardization.
Summary:
- Experiments in dogs and rabbits revealed that BL has a low immunogenic effect.
- Parenteral administration of BL led to the production of specific antibodies, detectable by Ouchterlony test, direct hemagglutination, and immunodiffusion.
- Gel filtration and immunodiffusion were used to determine BL's fraction composition.
Impact:
- The study highlights the necessity of considering BL's specific antibody induction in its therapeutic development.
- Immunochemical techniques offer a viable method for standardizing BL preparations.
- Findings contribute to the rational development of BL as a pharmacological agent.