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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

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Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI spectrometry is widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.
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Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
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Mass Spectrometry: Complex Analysis01:21

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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Mass Spectrometers01:16

Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Updated: May 17, 2025

Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples
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TEMI: tissue-expansion mass-spectrometry imaging.

Hua Zhang1, Lang Ding2,3, Amy Hu2

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We developed tissue-expansion mass spectrometry imaging (TEMI) for high-resolution spatial mapping of biomolecules. TEMI reveals metabolic differences in tumors and advances multi-omics research.

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Area of Science:

  • Biomolecular analysis
  • Spatial omics
  • Mass spectrometry imaging

Background:

  • Accurate spatial distribution mapping of biomolecules is vital for understanding physiological functions in multicellular organisms.
  • High-throughput in situ biomolecule mapping requires high scanning speed, spatial resolution, and chemical sensitivity for basic and medical research.

Purpose of the Study:

  • To develop a novel tissue-expansion method compatible with matrix-assisted laser desorption/ionization mass spectrometry imaging (TEMI).
  • To achieve single-cell spatial resolution for biomolecule profiling without compromising voxel throughput.
  • To enable comprehensive profiling of diverse biomolecules, including lipids, metabolites, peptides, and N-glycans.

Main Methods:

  • Integration of a tissue-expansion technique with matrix-assisted laser desorption/ionization mass spectrometry imaging (TEMI).
  • High-throughput scanning capabilities enabling single-cell resolution.
  • Profiling of multiple classes of biomolecules simultaneously.

Main Results:

  • TEMI successfully achieved single-cell spatial resolution for biomolecule mapping.
  • The method enabled the profiling of hundreds of biomolecules across various mammalian tissues.
  • Application of TEMI uncovered significant metabolic heterogeneity within tumors.

Conclusions:

  • TEMI is a powerful tool for high-throughput, high-resolution spatial mapping of biomolecules.
  • The technique facilitates the advancement of spatial multi-omics profiling in biological and medical research.
  • TEMI's adaptability makes it broadly applicable for diverse research applications, including cancer research.