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Related Experiment Video

Updated: Jun 21, 2026

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

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Published on: August 6, 2014

Temporal and spatial omics technologies for 4D profiling.

David E Reynolds¹, Yoon Ho Roh^2,3, Daniel Oh¹

¹Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, USA.

|April 22, 2025

Summary

Capturing cell dynamics requires advanced spatial omics. This perspective explores temporal and 3D spatial omics fusion and 4D profiling for deeper biological insights.

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Last Updated: Jun 21, 2026

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

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Published on: August 6, 2014

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Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Area of Science:

Molecular biology
Cell biology
Biotechnology

Background:

Cells possess unique surface and intracellular molecular profiles crucial for biological responses.
Understanding cellular dynamics in growth, development, and disease is limited by challenges in capturing temporal and spatial characteristics.
Existing omics techniques struggle to provide a comprehensive view of living cells over time and space.

Purpose of the Study:

To review techniques for temporal and 2D/3D spatial omics analyses.
To highlight the potential of fusing these techniques for understanding cellular dynamics.
To explore the emerging field of four-dimensional (4D) profiling in biological systems.

Main Methods:

Discussion of temporal omics techniques.
Analysis of 2D and 3D spatial omics methodologies.
Exploration of the integration of temporal and spatial omics data.
Review of advancements and challenges in 4D profiling.

Main Results:

The fusion of temporal and spatial omics offers powerful insights into cell dynamics and diversity.
Four-dimensional profiling, integrating time with 3D spatial omics, represents a promising frontier.
Current technologies face challenges in precise temporal and spatial resolution.
Further technological development is needed to fully realize the potential of 4D profiling.

Conclusions:

Integrating temporal and spatial omics is key to unlocking a deeper understanding of cellular behavior.
Four-dimensional profiling holds significant promise for future biological research.
Addressing current technological gaps will accelerate advancements in single-cell analysis.