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Comprehensive transcriptome of muscle development in Sichuan white rabbit
Xiangyu Zhang1,2, Kai Zhang3, Dengping Huang1,2
1Sichuan Academy of Science Academy, Chengdu, 610066, China.
BMC Genomic Data
|April 23, 2025
Summary
This study reveals the complex gene regulatory network controlling muscle development in Sichuan white rabbits. It identifies key messenger RNAs (mRNAs), microRNAs (miRNAs), and long non-coding RNAs (lncRNAs) involved in myogenesis for improved breeding.
Area of Science:
- Animal Genetics
- Molecular Biology
- Developmental Biology
Background:
- The Sichuan white rabbit is a high-yield domestic breed.
- Muscle development mechanisms in this breed remain unclear.
- Understanding myogenesis is crucial for livestock improvement.
Purpose of the Study:
- To comprehensively analyze the transcriptome of Sichuan white rabbits during muscle development.
- To elucidate the regulatory network governing myogenesis.
- To identify key genes and non-coding RNAs involved in muscle growth.
Main Methods:
- Generation of transcriptome datasets (mRNAs, miRNAs, lncRNAs) across three developmental stages.
- Differential gene expression analysis.
- Functional enrichment and interaction network analysis.
Main Results:
- Identified 2,995 differentially expressed (DE) genes, 1,211 DE-lncRNAs, and 305 DE-miRNAs.
- Functional analysis linked DE mRNAs and miRNAs to muscle growth, development, and regeneration.
- Uncovered a complex lncRNA-mRNA regulatory network, particularly between 21-27 days of development.
Conclusions:
- Provided a comprehensive transcriptomic overview of Sichuan white rabbit muscle development.
- Identified key DE genes, miRNAs, and lncRNAs offering insights into molecular mechanisms.
- Highlighted the potential role of non-coding RNAs in muscle biology and breeding improvement.
