Related Experiment Video

Updated: May 10, 2025

Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples
07:03

Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples

Published on: October 2, 2020

4.3K

SNORA47 affects stemness and chemotherapy sensitivity via EBF3/RPL11/c-Myc axis in luminal A breast cancer

Qilin Han1,2, Ying Zhou1, Zixian Dong1

  • 1Department of Breast Surgery, the First Hospital of China Medical University, 155 Nanjing North Street, Heping District, Shenyang, Liaoning, 110001, China.

Molecular Medicine (Cambridge, Mass.)
|April 23, 2025
PubMed

Insights

High SNORA47 expression in Luminal A breast cancer is linked to poor survival and chemotherapy resistance. This small nucleolar RNA drives cancer stemness by regulating the c-Myc oncogene via the EBF3-RPL11 pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genomics

Background:

  • Chemotherapy resistance in breast cancer impacts patient prognosis.
  • Breast cancer stem cells (BCSCs) are implicated in chemotherapy resistance.
  • SNORA47, a small nucleolar RNA, is uncharacterized in breast cancer but known in lung cancer.

Purpose of the Study:

  • To investigate the role of SNORA47 in breast cancer, particularly Luminal A subtype.
  • To explore the association between SNORA47 expression and patient survival and tumor characteristics.
  • To elucidate the molecular mechanism by which SNORA47 influences breast cancer stemness and drug sensitivity.

Main Methods:

  • Analysis of SNORA47 expression in The Cancer Genome Atlas (TCGA) database for breast cancer patients.
  • Correlation analysis of SNORA47 expression with clinical parameters like TNM stage.
  • In vitro and in vivo experiments to assess the impact of SNORA47 on breast cancer stemness and tumor sensitivity.
  • Investigation of molecular interactions involving SNORA47, EBF3, RPL11, and c-Myc.

Main Results:

  • High SNORA47 expression correlated with unfavorable survival outcomes in Luminal A breast cancer patients.
  • Elevated SNORA47 levels were associated with advanced TNM stage in Luminal A breast cancer.
  • SNORA47 demonstrated a strong link to breast cancer stemness and tumor sensitivity both in vitro and in vivo.
  • SNORA47 facilitates c-Myc regulation by interacting with EBF3 and influencing RPL11 translocation.

Conclusions:

  • SNORA47 is a potential biomarker for poor prognosis in Luminal A breast cancer.
  • The SNORA47-EBF3-RPL11 axis plays a critical role in regulating c-Myc and promoting breast cancer stemness.
  • Targeting the SNORA47 pathway offers a potential therapeutic strategy to overcome chemotherapy resistance in breast cancer.
Keywords:
Chemotherapy sensitivityEBF3Luminal A breast cancerSNORA47Stemness

More Related Videos

In vitro Enrichment of Ovarian Cancer Tumor-initiating Cells11:37

In vitro Enrichment of Ovarian Cancer Tumor-initiating Cells

Published on: February 18, 2015

13.7K
Murine Model for Non-invasive Imaging to Detect and Monitor Ovarian Cancer Recurrence08:55

Murine Model for Non-invasive Imaging to Detect and Monitor Ovarian Cancer Recurrence

Published on: November 2, 2014

12.2K

Related Concept Videos

The Nucleolus02:55

The Nucleolus

The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
8.6K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
3.8K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.6K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.3K