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Visualization and Analysis of Blood Flow and Oxygen Consumption in Hepatic Microcirculation: Application to an Acute Hepatitis Model
10:40

Visualization and Analysis of Blood Flow and Oxygen Consumption in Hepatic Microcirculation: Application to an Acute Hepatitis Model

Published on: August 5, 2012

Lactate dehydrogenase-6. A biochemical sign of serious hepatic circulatory disturbance

P L Wolf

    Archives of Internal Medicine
    |August 1, 1985
    PubMed

    Insights

    Researchers identified a novel lactate dehydrogenase (LD) isozyme band in patients with arteriosclerotic cardiovascular disease. This heat-stable isozyme, composed of M subunits, may indicate a modified LD-5 or alcohol dehydrogenase.

    Area of Science:

    • Biochemistry
    • Clinical Chemistry
    • Cardiology

    Background:

    • Lactate dehydrogenase (LD) isozymes are crucial biomarkers in diagnosing various diseases.
    • Arteriosclerotic cardiovascular disease can lead to congestive heart failure and organ congestion.
    • Previous research has characterized the standard LD isozyme profiles.

    Purpose of the Study:

    • To investigate the presence and characteristics of an additional LD isozyme band in patients with congestive heart failure.
    • To determine the clinical significance and biochemical properties of this novel isozyme.

    Main Methods:

    • Agarose gel isozyme electrophoresis was used to separate and identify LD isozymes.
    • Biochemical analyses were performed to assess the isozyme's heat stability, subunit composition, and binding properties.

    Main Results:

    • An additional LD isozyme band, cathodic to LD-5, was detected in seven patients.
    • The novel isozyme exhibited extreme heat stability and was not immunoglobulin bound.
    • Biochemical analysis revealed the isozyme contained only M subunits, suggesting it may be a modified LD-5 or alcohol dehydrogenase.

    Conclusions:

    • The identified LD isozyme may serve as a potential indicator in patients with severe arteriosclerotic cardiovascular disease and congestive heart failure.
    • Further research is warranted to elucidate the exact nature and clinical utility of this heat-stable, M-subunit-only isozyme.

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